EVALUATION OF ACTION ESTROGEN AND THYROID HORMONE ACTION UPON NONCODING RNA EXPRESSION IN OSTEOBLASTIC CELLS DERIVED OF ADIPOSE TISSUE

• Grant number: 14/16406-9
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2015 - November 30, 2017
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Celia Regina Nogueira
• Grantee: Celia Regina Nogueira
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Claudia Aparecida Rainho ; Maria Do Carmo Salazar Velez Roque da Fonseca

Abstract

The bone tissue is extremely complex and is regulated by systemic and local factors. It presents considerable metabolic activity including the removal of mineralized bone through the osteoclasts followed by the formation of bone matrix by the osteoblasts. The association of T3 and E2 might lead to a complex response to the bone tissue activity and T3 produces effects upon bone resorption as well as bone formation. As for E2, when in low level, it might lead to osteoporosis whereas in normal state it guarantees cytokine suppression such as RANK, RANKL and OPG which actively participate in bone remodeling. Therefore, various studies aim at verifying the hormone action upon bone metabolism and within them there are groups isolating mesenchymal stem cell from human adipose tissue and diferentiating into osteoblasts. The differentiation might be triggered by several factors including the expression of some specific genes that are related to various gene expression regulating mechanisms such as small non-codingRNAs (ncRNAs), micro RNAs (miRNAs) and small interfering RNAs (siRNA). Hence, the identification of small RNAs involved in the osteoblast differentiation from the quantitative transcriptome sequencing might lead to answer some questions about the complex bone metabolism. Thus, the present study allows the analysis both the differentiation of osteoblasts and the profile of transcriptome during the progression to osteoclastogenesis stimulated by thyroid hormone and estrogen. It also identifies small RNAs with potential applicability to the reversion or maintenance of such processes in a field that is eminent within bone biology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTRO OLIMPIO, REGIANE MARQUES; FONTES MORETTO, FERNANDA CRISTINA; DE SIBIO, MARIA TERESA; DE OLIVEIRA, MIRIANE; MATHIAS, LUCAS SOLLA; GONCALVES, BIANCA MARIANI; DEPRA, IGOR CARVALHO; TILLI, HELENA PAIM.; RODRIGUES, BRUNA MORETTO; SARAIVA, PATRICIA PINTO; et al. The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts. Life Sciences, v. 231, AUG 15 2019. (14/16406-9)

BARNEZE COSTA, SARAH MARIA; FELTRAN, GEORGIA SILVA; NAMBA, VICKELINE; SILVA, TABATA MARILDA; HALLUR, RAGHAVENDRA LAKSHMANA SHETTY; SARAIVA, PATRICIA PINTO; ZAMBUZZI, WILLIAN FERNANDO; NOGUEIRA, CELIA REGINA. Infraphysiological 17 beta-estradiol (E2) concentration compromises osteoblast differentiation through Src stimulation of cell proliferation and ECM remodeling stimulus. Molecular and Cellular Endocrinology, v. 518, DEC 1 2020. (14/15529-0, 14/22689-3, 14/16406-9)