| Texto completo | |
| Autor(es): |
Lee, Aejin
[1]
;
Pontin, Mariana C. F.
[2]
;
Kosmerl, Erica
[3]
;
Jimenez-Flores, Rafael
[3]
;
Moretti, Debora B.
[2]
;
Ziouzenkova, Ouliana
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Ohio State Univ, Dept Human Nutr, Campbell Hall, 1787 Neil Ave, Columbus, OH 43210 - USA
[2] Univ Sao Paulo, Dept Anim Sci, Luiz de Queiroz Coll Agr, Av Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil
[3] Ohio State Univ, Dept Food Sci & Technol, Parker Food Sci & Technol, 2015 Fyffe Rd, Columbus, OH 43210 - USA
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF DAIRY SCIENCE; v. 102, n. 10, p. 8614-8621, OCT 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Bovine colostrum (BC) has been used for nutra-ceutical purposes for animals and humans. Bovine colostrum is a complex heterogeneous product and its antimicrobial activity, antioxidant potential, and growth factors can vary depending on age and species of the cow as well as their environment. Bovine colostrum preparation in skimmed or whey fractions can also alter properties of BC. Our goal was to compare cumulative anti-inflammatory, antioxidant, and adipogenic properties of natural (whole) versus whey BC. We compared properties of whole and whey BC in 3T3-L1 preadipocytes permanently transfected with reporters responding to changes in inflammatory (Nf kappa bRE/green fluorescent protein), anti-inflammatory (Nrf2/YFP), and adipogenic (Fabp4/cyan fluorescent protein) star tus in cells. Interleukin-6 secretion in these cells was measured by ELISA. Whole and whey BC induce IL-6 secretion from 3T3-L1 fibroblasts; however, whey preparation stimulated less IL-6 secretion. Cumulative inflammatory nuclear factor (NF)kappa B activation in the presence of lipopolysaccharide was reduced by both whole (-27%) and whey BC (-22%) compared with lipopolysaccharide-treated cells (100%). Treatment with whole BC was more effective in the reduction of NF kappa B activation compared with whey BC and occurred in a dose-dependent manner. In consonance with decreased NF kappa B activation, the Nrf2 promoter activity was also reduced in response to whole (-27%) and whey (-13%) treatments compared with nontreated cells (100%). Whole and whey BC suppressed adipogenesis, measured as induction of Fabp4, by -27 and -13%, respectively, compared with nontreated 3T3-L1 fibroblasts (100%). Our results showed distinct differences in properties of whey and whole BC that could be used to attain reduced adipogenic or cumulative inflammatory responses. (AU) | |
| Processo FAPESP: | 14/14937-7 - Fatores antioxidantes do colostro bovino na proteção celular |
| Beneficiário: | Débora Botéquio Moretti |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 17/02915-7 - Efeito do colostro bovino na expressão de NFkB |
| Beneficiário: | Mariana Caroline Furian Pontin |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Mestrado |