| Texto completo | |
| Autor(es): |
Orfali, Raquel Leao
[1]
;
Yamada Yoshikawa, Fabio Seiti
[1]
;
da Silva Oliveira, Luanda Mara
[1]
;
Pereira, Natalli Zanete
[1]
;
de Lima, Josenilson Feitosa
[1]
;
Leuzzi Ramos, Yasmim Alefe
[1]
;
da Silva Duarte, Alberto Jose
[1]
;
Sato, Maria Notomi
[1]
;
Aoki, Valeria
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med FMUSP, Lab Dermatol & Immunodeficiencies LIM 56, Dept Dermatol, Sao Paulo, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC REPORTS; v. 9, SEP 11 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Staphylococcus aureus colonizes the skin of atopic dermatitis (AD) individuals, but the impact of its enterotoxins on the chronic activation of CD4(+)T cells demands further analysis. We aimed to analyze the CD4(+)T cell anergy profile and their phenotypic and functional features through differential expression of cellular activation markers, cytokine production and response to staphylococcal enterotoxin A (SEA). A panel of 84 genes relevant to T cell anergy was assessed by PCR array in FACS-sorted CD4(+)T cells, and the most prominent genes were validated by RT-qPCR. We evaluated frequencies of circulating CD4(+)T cells secreting single or multiple (polyfunctional) cytokines (IL-17A, IL-22, TNF, IFN-gamma, and MIP-1 beta) and expression of activation marker CD38 in response to SEA stimulation by flow cytometry. Our main findings indicated upregulation of anergy-related genes (EGR2 and IL13) promoted by SEA in AD patients, associated to a compromised polyfunctional response particularly in CD4(+)CD38(+)T cells in response to antigen stimulation. The pathogenic role of staphylococcal enterotoxins in adult AD can be explained by their ability to downmodulate the activated effector T cell response, altering gene expression profile such as EGR2 induction, and may contribute to negative regulation of polyfunctional CD4(+)T cells in these patients. (AU) | |
| Processo FAPESP: | 16/24161-1 - Caracterização proteômica de exossomas do plasma e de fibroblastos dérmicos na dermatite atópica do adulto |
| Beneficiário: | Raquel Leão Orfali |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/25645-7 - Avaliação fenotípica e funcional de células dendríticas inflamatórias e de células T na dermatite atópica do adulto |
| Beneficiário: | Valeria Aoki |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |