| Full text | |
| Author(s): |
Orfali, Raquel Leao
[1]
;
Yamada Yoshikawa, Fabio Seiti
[1]
;
da Silva Oliveira, Luanda Mara
[1]
;
Pereira, Natalli Zanete
[1]
;
de Lima, Josenilson Feitosa
[1]
;
Leuzzi Ramos, Yasmim Alefe
[1]
;
da Silva Duarte, Alberto Jose
[1]
;
Sato, Maria Notomi
[1]
;
Aoki, Valeria
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med FMUSP, Lab Dermatol & Immunodeficiencies LIM 56, Dept Dermatol, Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 9, SEP 11 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Staphylococcus aureus colonizes the skin of atopic dermatitis (AD) individuals, but the impact of its enterotoxins on the chronic activation of CD4(+)T cells demands further analysis. We aimed to analyze the CD4(+)T cell anergy profile and their phenotypic and functional features through differential expression of cellular activation markers, cytokine production and response to staphylococcal enterotoxin A (SEA). A panel of 84 genes relevant to T cell anergy was assessed by PCR array in FACS-sorted CD4(+)T cells, and the most prominent genes were validated by RT-qPCR. We evaluated frequencies of circulating CD4(+)T cells secreting single or multiple (polyfunctional) cytokines (IL-17A, IL-22, TNF, IFN-gamma, and MIP-1 beta) and expression of activation marker CD38 in response to SEA stimulation by flow cytometry. Our main findings indicated upregulation of anergy-related genes (EGR2 and IL13) promoted by SEA in AD patients, associated to a compromised polyfunctional response particularly in CD4(+)CD38(+)T cells in response to antigen stimulation. The pathogenic role of staphylococcal enterotoxins in adult AD can be explained by their ability to downmodulate the activated effector T cell response, altering gene expression profile such as EGR2 induction, and may contribute to negative regulation of polyfunctional CD4(+)T cells in these patients. (AU) | |
| FAPESP's process: | 16/24161-1 - Protemic characterization of exosomes from plasma and dermal fibroblasts in adults with atopic dermatitis |
| Grantee: | Raquel Leão Orfali |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/25645-7 - Evaluation of inflammatory dendritic cells and T cells in adults with atopic dermatitis |
| Grantee: | Valeria Aoki |
| Support Opportunities: | Regular Research Grants |