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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Staphylococcal enterotoxins modulate the effector CD4(+)T cell response by reshaping the gene expression profile in adults with atopic dermatitis

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Author(s):
Orfali, Raquel Leao [1] ; Yamada Yoshikawa, Fabio Seiti [1] ; da Silva Oliveira, Luanda Mara [1] ; Pereira, Natalli Zanete [1] ; de Lima, Josenilson Feitosa [1] ; Leuzzi Ramos, Yasmim Alefe [1] ; da Silva Duarte, Alberto Jose [1] ; Sato, Maria Notomi [1] ; Aoki, Valeria [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Med FMUSP, Lab Dermatol & Immunodeficiencies LIM 56, Dept Dermatol, Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, SEP 11 2019.
Web of Science Citations: 0
Abstract

Staphylococcus aureus colonizes the skin of atopic dermatitis (AD) individuals, but the impact of its enterotoxins on the chronic activation of CD4(+)T cells demands further analysis. We aimed to analyze the CD4(+)T cell anergy profile and their phenotypic and functional features through differential expression of cellular activation markers, cytokine production and response to staphylococcal enterotoxin A (SEA). A panel of 84 genes relevant to T cell anergy was assessed by PCR array in FACS-sorted CD4(+)T cells, and the most prominent genes were validated by RT-qPCR. We evaluated frequencies of circulating CD4(+)T cells secreting single or multiple (polyfunctional) cytokines (IL-17A, IL-22, TNF, IFN-gamma, and MIP-1 beta) and expression of activation marker CD38 in response to SEA stimulation by flow cytometry. Our main findings indicated upregulation of anergy-related genes (EGR2 and IL13) promoted by SEA in AD patients, associated to a compromised polyfunctional response particularly in CD4(+)CD38(+)T cells in response to antigen stimulation. The pathogenic role of staphylococcal enterotoxins in adult AD can be explained by their ability to downmodulate the activated effector T cell response, altering gene expression profile such as EGR2 induction, and may contribute to negative regulation of polyfunctional CD4(+)T cells in these patients. (AU)

FAPESP's process: 16/24161-1 - Protemic characterization of exosomes from plasma and dermal fibroblasts in adults with atopic dermatitis
Grantee:Raquel Leão Orfali
Support Opportunities: Regular Research Grants
FAPESP's process: 14/25645-7 - Evaluation of inflammatory dendritic cells and T cells in adults with atopic dermatitis
Grantee:Valeria Aoki
Support Opportunities: Regular Research Grants