Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

Autor(es)

Bensaoud, Chaima ^{[1, 2]} ; Aounallah, Hajer ^[1] ; Sciani, Juliana Mozer ^{[3, 4]} ; Faria, Fernanda ^[3] ; Chudzinski-Tavassi, Ana Marisa ^[3] ; Bouattour, Ali ^[1] ; M'ghirbi, Youmna ^[1]

Afiliação do(s) autor(es)

^[1] Univ Tunis El Manar, Inst Pasteur Tunis, Serv Entomol Med, LR11IPT03, Tunis 1002 - Tunisia

^[2] Czech Acad Sci, Biol Ctr, Inst Parasitol, Ceske Budejovice 37005 - Czech Republic

^[3] Inst Butantan, Lab Biol Mol, Av Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

^[4] Univ Sao Francisco, Lab Multidisciplinar Pesquisa, Av Sao Francisco de Assis 218, BR-12916900 Paulista, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 20, n. 1 AUG 27 2019.
• Citações Web of Science: 0

Resumo

BackgroundThe hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species.ResultsWe reported the array of 1111 proteins identified in the salivary glands of H. dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species.ConclusionThis investigation represents the first proteomic study of H. dromedarii salivary glands. Our results shed light on the differences between the composition of H. dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs