Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery

Autor(es)

Gallo, Jose Renato B. ^[1] ; Maschio-Signorini, Larissa B. ^[2] ; Cabral, Celso R. B. ^[3] ; de Campos Zuccari, Debora A. P. ^[4] ; Nogueira, Mauricio L. ^[5] ; Bozola, Antonio R. ^[6] ; Cury, Patricia M. ^[7] ; Vidotto, Alessandra ^[8]

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose Do Rio Preto FAMERP, Med Postgrad Program, Dept Plast Surg, Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose Do Rio Preto FAMERP, Mol Invest Canc Lab LIMC, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Fed Amazonas UFAM, Dept Stat, Manaus, Amazonas - Brazil

^[4] Fac Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

^[5] Fac Med Sao Jose Do Rio Preto FAMERP, Virol Lab, Dept Dermatol Infect & Parasit Dis, Sao Jose Do Rio Preto, SP - Brazil

^[6] Fac Med Sao Jose Do Rio Preto FAMERP, Dept Plast Surg, Sao Jose Do Rio Preto, SP - Brazil

^[7] FACERES, Med Course, Fac Med, Sao Jose Do Rio Preto, SP - Brazil

^[8] Fac Med Sao Jose Do Rio Preto FAMERP, Multiuser Lab, Ave Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN; v. 7, n. 8 AUG 2019.
• Citações Web of Science: 0

Resumo

Background: Chronic inflammation during morbid obesity significantly alters cutaneous tissue. Large weight loss achieved after bariatric surgery minimizes or halts damage caused by metabolic syndrome, but further deteriorates the clinical condition of skin. Postbariatric skin flaccidity produces major difficulties to plastic surgery. In this study, we analyzed differences in protein composition of the skin between patients with morbid obesity and those after large weight loss and established correlations between differentially expressed proteins and clinical characteristics of postbariatric skin tissue, to improve body contouring surgery techniques. Methods: Skin fragments were removed from the abdomen of 32 patients, who were allocated into 3 groups: morbidly obese, large weight loss without surgery, and postbariatric surgery. Samples were subjected to proteomic analysis, and the protein profiles of the groups were compared. Six differentially expressed proteins of clinical interest were validated by immunohistochemistry and statistical analysis. Results: Comparative analyses confirmed differences in protein profile of the skin between morbidly obese and large weight loss groups. A persistent increase in inflammatory markers such as haptoglobin was observed in all groups and decrease in the expression of collagen XIV, which regulates the physical properties of cutaneous tissue, was observed in the postbariatric group. Conclusions: High expression of haptoglobin associated with the decrease of Collagen XIV, vinculin, and periplakin in the groups after major weight losses, mainly postbariatric, confirm that the inflammatory lesion remains active in the skin and causes changes in its structural organization, with serious repercussions on its clinical characteristics and physical properties. (AU)

• Processo FAPESP: 04/14846-0 - Rede de proteoma do estado de São Paulo
• Beneficiário: Fabio Cesar Gozzo
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA