Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by Candida albicans Resistant to Fluconazole

Autor(es)

Rimachi Hidalgo, Karem Janeth ^[1] ; Carmello, Juliana Cabrini ^[1] ; Jordao, Claudia Carolina ^[1] ; Barbugli, Paula Aboud ^[1] ; de Sousa Costa, Carlos Alberto ^[2] ; de Oliveira Mima, Ewerton Garcia ^[1] ; Pavarina, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, UNESP, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICALS; v. 12, n. 3 SEP 2019.
• Citações Web of Science: 0

Resumo

Background: It has been demonstrated that azole-resistant strains of Candida albicans have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant C. albicans (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log(10) and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by C. albicans resistant to fluconazole. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs