Extracellular Vesicles Shedding Promotes Melanoma Growth in Response to Chemotherapy

Autor(es)

de Sousa Andrade, Luciana Nogueira ^[1] ; Otake, Andreia Hanada ^[1] ; Braga Cardim, Silvia Guedes ^[1] ; da Silva, Felipe I. Lelis ^[1] ; Ikoma Sakamoto, Mariana Mari ^[1] ; Furuya, Tatiane Katsue ^[1] ; Uno, Miyuki ^[1] ; Pasini, Fatima Solange ^[1] ; Chammas, Roger ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Inst Canc Estado Sao Paulo, Ctr Invest Translac On, Ave Dr Arnaldo 251, BR-01246000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, OCT 9 2019.
• Citações Web of Science: 0

Resumo

Extracellular vesicles (EVs) are emerging as key players in intercellular communication. EVs can transfer biological macromolecules to recipient cells, modulating various physiological and pathological processes. It has been shown that tumor cells secrete large amounts of EVs that can be taken up by malignant and stromal cells, dictating tumor progression. In this study, we investigated whether EVs secreted by melanoma cells in response to chemotherapy modulate tumor response to alkylating drugs. Our findings showed that human and murine melanoma cells secrete more EVs after treatment with temozolomide and cisplatin. We observed that EVs shed by melanoma cells after temozolomide treatment modify macrophage phenotype by skewing macrophage activation towards the M2 phenotype through upregulation of M2-marker genes. Moreover, these EVs were able to favor melanoma re-growth in vivo, which was accompanied by an increase in Arginase 1 and IL10 gene expression levels by stromal cells and an increase in genes related to DNA repair, cell survival and stemness in tumor cells. Taken together, this study suggests that EVs shed by tumor cells in response to chemotherapy promote tumor repopulation and treatment failure through cellular reprogramming in melanoma cells. (AU)

• Processo FAPESP: 18/01366-2 - 7th Annual International Society for Extracellular Vesicles (ISEV) Conference
• Beneficiário: Luciana Nogueira de Sousa Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 14/03742-0 - Projeto temático em medicina translacional: nanopartículas que se ligam a receptores de lipoproteínas no tratamento da aterosclerose, do infarto agudo de miocárdio, do pós-transplante de coração, do câncer e da endometriose
• Beneficiário: Raul Cavalcante Maranhao
• Modalidade de apoio: Auxílio à Pesquisa - Temático