Protic Ionic Liquids as Cell-Disrupting Agents for the Recovery of Intracellular Carotenoids from Yeast Rhodotorula glutinis CCT-2186

Autor(es)

Mussagy, Cassamo U. ^[1] ; Santos-Ebinuma, Valeria C. ^[1] ; Gonzalez-Miquel, Maria ^[2] ; Coutinho, Joao A. P. ^[3] ; Pereira, Jorge F. B. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Rodovia Araraquara Jau, Km 01, BR-14800903 Araraquara, SP - Brazil

^[2] Univ Politecn Madrid, ETS Ingenieros Ind, Dept Ingn Quim Ind & Medio Ambiente, C Jose Gutierrez Abascal 2, E-28006 Madrid - Spain

^[3] Univ Aveiro, Dept Chem, CICECO Aveiro Inst Mat, P-3810193 Aveiro - Portugal

• Tipo de documento: Artigo Científico
• Fonte: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 7, n. 19, p. 16765-16776, OCT 7 2019.
• Citações Web of Science: 0

Resumo

Rhodotorula glutinis (R. glutinis) yeasts are natural sources of intracellular carotenoids such as beta-carotene, torularhodin, and torulene. Since these yeasts are constituted by a rigid cell-wall structure, the use of energy-saving and high-efficiency cell disruption procedures is critical for carotenoids recovery. A new technology using protic ionic liquids (PILs) was here evaluated as an alternative platform to permeabilize the R. glutinis cells and to improve the extraction of beta-carotene, torularhodin, and torulene. The cell disruption ability of 12 highly concentrated aqueous solutions of ammonium-based PILs was determined, evaluating the influence of the relative ion hydrophobicity, solid-liquid ratio, water content, and temperature. Carotenoid extraction yields increased with the hydrophobicity of the PILs (i.e., increase of alkyl chain length of the anion or cation), temperature (from 25 to 65 degrees C), and PIL concentration (from 75 to 90% v/v). Additionally, to demonstrate the potential of PILs in carotenoids recovery, solvent recycling and carotenoids polishing were carried out using a three-phase partitioning system. The results demonstrate that the use of PILs as cell-disrupting agents can be a simple, efficient, sustainable, and feasible method to recover intracellular carotenoids from microbial biomass. (AU)

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