Chemosensitizing Effect of Cernumidine Extracted from Solanum cernuum on Bladder Cancer Cells in Vitro

Autor(es)

Miranda, Mariza A. ^[1] ; Mondal, Arindam ^[2] ; Sachdeva, Mandip ^[2] ; Cabral, Hamilton ^[1] ; Neto, Youssef A. A. H. ^[1] ; Khan, Ikhlas ^[3] ; Groppo, Milton ^[4] ; McChesney, James D. ^[5] ; Bastos, Jairo K. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Florida A&M Univ, Coll Pharm & Pharmaceut Sci, Tallahassee, FL 32307 - USA

^[3] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, Oxford, MS 38677 - USA

^[4] Univ Sao Paulo, Fac Philosophy Sci & Literature Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[5] Ironstone Separat Inc, 147 Cty Rd 245, Etta, MS 38627 - USA

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. 16, n. 10 OCT 2019.
• Citações Web of Science: 0

Resumo

Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and the possible mechanism of action of the combination on bladder cancer cells. Cernumidine showed cytotoxicity and could sensitize bladder cancer cells to cisplatin. The combination of CER+cDDP inhibited cell migration on T24 cells. CER+cDDP down-regulated MMP-2/9 and p-ERK1/2, while it increased EGFR activity corroborating the observed cell migration inhibition. Down-regulation of Bcl-2 and up-regulation pro-apoptotic Bax and further depletion of the mitochondrial membrane potential (Delta psi m) indicates that mitochondria play a central role in the combination treatment inducing the mitochondrial signaling pathway of apoptosis in T24 cells. Our data showed that the alkaloid cernumidine is worthy of further studies as a chemosensitizing agent to be used in complementary chemotherapy. (AU)

• Processo FAPESP: 12/00715-7 - Solanum cernuum Vell: estudo fitoquímico, avaliação das atividades antiulcerogênica, antimicrobiana, citotóxica e obtenção de extrato seco por spray dryer
• Beneficiário: Mariza Abreu Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado