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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chemosensitizing Effect of Cernumidine Extracted from Solanum cernuum on Bladder Cancer Cells in Vitro

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Author(s):
Miranda, Mariza A. [1] ; Mondal, Arindam [2] ; Sachdeva, Mandip [2] ; Cabral, Hamilton [1] ; Neto, Youssef A. A. H. [1] ; Khan, Ikhlas [3] ; Groppo, Milton [4] ; McChesney, James D. [5] ; Bastos, Jairo K. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Florida A&M Univ, Coll Pharm & Pharmaceut Sci, Tallahassee, FL 32307 - USA
[3] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, Oxford, MS 38677 - USA
[4] Univ Sao Paulo, Fac Philosophy Sci & Literature Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[5] Ironstone Separat Inc, 147 Cty Rd 245, Etta, MS 38627 - USA
Total Affiliations: 5
Document type: Journal article
Source: CHEMISTRY & BIODIVERSITY; v. 16, n. 10 OCT 2019.
Web of Science Citations: 0
Abstract

Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and the possible mechanism of action of the combination on bladder cancer cells. Cernumidine showed cytotoxicity and could sensitize bladder cancer cells to cisplatin. The combination of CER+cDDP inhibited cell migration on T24 cells. CER+cDDP down-regulated MMP-2/9 and p-ERK1/2, while it increased EGFR activity corroborating the observed cell migration inhibition. Down-regulation of Bcl-2 and up-regulation pro-apoptotic Bax and further depletion of the mitochondrial membrane potential (Delta psi m) indicates that mitochondria play a central role in the combination treatment inducing the mitochondrial signaling pathway of apoptosis in T24 cells. Our data showed that the alkaloid cernumidine is worthy of further studies as a chemosensitizing agent to be used in complementary chemotherapy. (AU)

FAPESP's process: 12/00715-7 - Solanum cernuum Vell: Phytochemical study, evaluation of antiulcerogenic, antimicrobial, cytotoxic activities and attainment of dry extract by spray dryer
Grantee:Mariza Abreu Miranda
Support Opportunities: Scholarships in Brazil - Doctorate