Development of solid lipid nanoparticles as carrier system of glycoalkaloids of Solanum lycocarpum for bladder cancer therapy

• Grant number: 14/16727-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): April 01, 2015
• Effective date (End): October 31, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Priscyla Daniely Marcato Gaspari
• Grantee: Ivana Pereira Santos Carvalho
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Carcinoma of the urinary bladder (CB) is the second most common malignancy of the urinary tract. In Brazil there are about 3200 deaths per year resulting from this type of cancer. Currently, the main CB therapies include immunotherapy with BCG and chemotherapy. But the effectiveness of these therapies is low, have a high recurrence rate and several adverse effects. Thus, there is a demand for novel therapeutic strategies such as the use of compounds extracted from plants such as glycoalkaloids, solasonine and solamargine, extracted from the fruit of the plant Solanum lycocarpum. These glycoalkaloids have antioxidant and anticarcinogenic properties acting in different interesting molecular mechanisms for cancer therapy. However, intravesical administration of drugs in the treatment of CB is a challenge, since the drugs have low permeability in the urothelium, low residence time in the bladder, due to periodic bladder emptying and filling. Furthermore, several administrations are needed, which causes high incidences of infections and local pain. Thus, a strategy to overcome these challenges is the application of nanomedicine, especially, the sustained release systems. In this line, this project aims at the development and characterization of Solid Lipid Nanoparticles (SLN) as carrier systems of extract of S. lycocarpum, in order to obtain a nanostructured system for intravesical therapy of bladder cancer. The NLS will be prepared by microemulsion and sonication method. The particles will be characterized regarding the size and zeta potential by photon correlation spectroscopy, morphology by atomic force microscopy and crystallinity by differential scanning calorimetry. In addition, the encapsulation efficiency and the mucoadhesive property of the NLS will be evaluated. The effectiveness of the developed nanostructured systems will be evaluated for cytotoxicity assays in vitro in cells of bladder cancer using 2D and 3D cell culture model. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, I. P. S.; MIRANDA, M. A.; SILVA, L. B.; CHRYSOSTOMO-MASSARO, T. N.; PASCHOAL, J. A. R.; BASTOS, J. K.; MARCATO, P. D.. IN VITRO Anticancer Activity and Physicochemical Properties of SOLANUM LYCOCARPUM Alkaloidic Extract Loaded in Natural Lipid-Based Nanoparticles. COLLOID AND INTERFACE SCIENCE COMMUNICATIONS, v. 28, p. 5-14, JAN 2019. (14/50928-2, 14/16727-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARVALHO, Ivana Pereira Santos. Development of solid lipid nanoparticles in loading alcaloídico extract of Solanum Lycocarpum and biological evaluation in vitro in bladder cancer cells. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.