Development and validation of an analytical method for the determination of 17 beta-estradiol residues in muscle of tambaqui (Colossoma macropomum Cuvier, 1818) by LC-MS/MS and its application in samples from a fish sexual reversion study

Autor(es)

Hoga, Celia A. ^[1] ; Reche, Karine V. G. ^[2] ; Almeida, Fernanda L. ^[3] ; Reis, Vanessa R. ^[3] ; Cordeiro, Rafaelle P. ^[1] ; Anadon, Arturo ^[4] ; Reyes, Felix G. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, UNICAMP, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Microbiot Lab Anal, Ave Santa Isabel 2116, BR-13084643 Campinas, SP - Brazil

^[3] Embrapa Amazonia Ocident, Rodovia 010 Km 29, Cx Postal 319, BR-69010970 Manaus, Amazonas - Brazil

^[4] Univ Complutense Madrid, Fac Vet Med, Dept Pharmacol & Toxicol, E-28040 Madrid - Spain

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1128, n. SI OCT 1 2019.
• Citações Web of Science: 0

Resumo

Tambaqui (Colossoma macropomum Cuvier, 1818) is the main native fish species farmed in Brazil, and 17 beta-estradiol (E-2) is a natural steroid hormone commonly used for the production of female fish monosex population, which, in tambaqui, shows a higher growth rate than the male. Thus, to assess whether the fish meat of treated tambaqui contains hormonal residue levels, a high-performance liquid chromatography coupled with electro-spray tandem mass spectrometry (LC-MS/MS) method for the determination of E-2 residues in fish muscle was developed and validated. A QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) dispersive solid phase extraction method was used for the sample preparation. The chromatographic separation was performed in a Poroshel EC-18 reverse phase column. The mobile phase was a mixture of acetonitrile with 0.01% ammonium hydroxide (A) and water with 0.01% ammonium hydroxide (B). The ratio of A:B phases was 60:40 (v/v) used in an isocratic mode. The method validation was performed according to Commission Decision 2002/657/EC and Veterinary International Conference Harmonization (VICH GL49). Since matrix effects were observed, matrixmatched analytical curves are recommended for quantitation. The linearity, selectivity, intraday and interday precision, accuracy, decision limit, detection capability, and detection and quantitation limits of the method are reported. The limits of detection and quantitation were 0.3 ng/g and 1.0 ng/g, respectively. At these limits and slaughtering fish 7 months after the end of the treatment, the muscle of tambaqui did not show detectable hormone residue level. Thus, consumption of tambaqui edible tissue from fish treated with E2 for the purpose of sexual reversion is unlikely to represent a risk associated with the exposure of human subjects as residue levels of this hormone are not detected in the fish muscle. (AU)

• Processo FAPESP: 13/50452-5 - Veterinary drugs: development and validation of bioanalytical methods using LC-MS/MS and MALDI-TOF-MS and its applications in sanitary surveillance and the development of fish farming in Brazil
• Beneficiário: Felix Guillermo Reyes Reyes
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE