Brazilian-adapted soybean Bradyrhizobium strains uncover IS elements with potential impact on biological nitrogen fixation

Autor(es)

Barros-Carvalho, Gesiele Almeida ^{[1, 2, 3]} ; Hungria, Mariangela ^[4] ; Lopes, Fabricio Martins ^[5] ; Van Sluys, Marie-Anne ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, GaTE Lab, 277 Matao St, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Bot, 277 Matao St, BR-05508090 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Matemat & Estat, 1010 Matao St, BR-05508090 Sao Paulo, SP - Brazil

^[4] Embrapa Soja, CP 231, BR-86001970 Londrina, PR - Brazil

^[5] Univ Tecnol Fed Parana, 1640 Alberto Carazzai Ave, BR-86300000 Cornelio Procopio, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 366, n. 11 JUN 2019.
• Citações Web of Science: 0

Resumo

Bradyrhizobium diazoefficiens CPAC 7 and Bradyrhizobium japonicum CPAC 15 are broadly used in commercial inoculants in Brazil, contributing to most of the nitrogen required by the soybean crop. These strains differ in their symbiotic properties: CPAC 7 is more efficient in fixing nitrogen, whereas CPAC 15 is more competitive. Comparative genomics revealed many transposases close to genes associated with symbiosis in the symbiotic island of these strains. Given the importance that insertion sequences (IS) elements have to bacterial genomes, we focused on identifying the local impact of these elements in the genomes of these and other related Bradyrhizobium strains to further understand their phenotypic differences. Analyses were performed using bioinformatics approaches. We found IS elements disrupting and inserted at regulatory regions of genes involved in symbiosis. Further comparative analyses with 21 Bradyrhizobium genomes revealed insertional polymorphism with distinguishing patterns between B. diazoefficiens and B. japonicum lineages. Finally, 13 of these potentially impacted genes are differentially expressed under symbiotic conditions in B. diazoefficiens USDA 110. Thus, IS elements are associated with the diversity of Bradyrhizobium, possibly by providing mechanisms for natural variation of symbiotic effectiveness. (AU)

• Processo FAPESP: 08/52074-0 - Sugarcane genome sequence: plant transposable elements are active contributors to gene structure variation, regulation and function
• Beneficiário: Marie-Anne Van Sluys
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático