Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

Autor(es)

Proenca, Camila A. ^[1] ; Freitas, Tayane A. ^[1] ; Baldo, Thaisa A. ^[1] ; Materon, Elsa M. ^{[2, 1]} ; Shimizu, Flavio M. ^{[2, 3]} ; Ferreira, Gabriella R. ^[4] ; Soares, Frederico L. F. ^{[1, 5]} ; Faria, Ronaldo C. ^[1] ; Oliveira Jr, Osvaldo N.

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Chem Dept, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13560970 Sao Carlos, SP - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil

^[4] Univ Sao Paulo, Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

^[5] Univ Fed Parana, Chem Dept, BR-81531980 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BEILSTEIN JOURNAL OF NANOTECHNOLOGY; v. 10, p. 2171-2181, NOV 6 2019.
• Citações Web of Science: 0

Resumo

Diagnosis of cancer using electroanalytical methods can be achieved at low cost and in rapid assays, but this may require the combination with data treatment for determining biomarkers in real samples. In this paper, we report an immunomagnetic nanoparticle-based microfluidic sensor (IN mu-SPCE) for the amperometric detection of the prostate-specific antigen (PSA) biomarker, the data of which were treated with information visualization methods. The IN mu-SPCE consists of eight working electrodes, reference and counter electrodes. On the working electrodes, magnetic nanoparticles with secondary antibodies with the enzyme horseradish peroxidase were immobilized for the indirect detection of PSA in a sandwich-type procedure. Under optimal conditions, the immunosensor could operate within a wide range from 12.5 to 1111 fg.L-1, with a low detection limit of 0.062 fg.L-1. Multidimensional projections combined with feature selection allowed for the distinction of cell lysates with different levels of PSA, in agreement with results from the traditional enzyme-linked immunosorbent assay. The approaches for immunoassays and data processing are generic, and therefore the strategies described here may provide a simple platform for clinical diagnosis of cancers and other types of diseases. (AU)

• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/00991-5 - Estudo da Interação de Enzimas de Detoxificação com Fármacos Quimioterápicos e sua relação com a Quimiorresistência
• Beneficiário: Elsa María Materón Vásques
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado