Effects of ghrelin supplementation on the acute phase of Chagas disease in rats

Autor(es)

Silva, Ferdinando de Paula ^[1] ; Bronzon da Costa, Cassia Mariana ^[1] ; Pereira, Luiz Miguel ^[1] ; Silva Lessa, Diego Fernando ^[1] ; Pitol, Dimitrius Leonardo ^[2] ; Mardegan Issa, Joao Paulo ^[2] ; do Prado Junior, Jose Clovis ^[1] ; Carraro Abrahao, Ana Amelia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, BR-14040903 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Morphol Physiol & Basic Pathol, BR-14040904 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITES & VECTORS; v. 12, n. 1 NOV 9 2019.
• Citações Web of Science: 0

Resumo

Background Trypanosoma cruzi is the causative agent of Chagas disease, which is endemic to subtropical and tropical Americas. The disease treatment remains partially ineffective, involving therapies directed to the parasite as well as palliative strategies for the clinical manifestations. Therefore, novel candidates for disease control are necessary. Additionally, strategies based on parasite inhibition via specific targets and application of compounds which improve the immune response against the disease is welcomed. Ghrelin is a peptide hormone pointed as a substance with important cardioprotective, vasodilatory, anti-apoptotic, anti-oxidative and immune modulatory functions. The aims of this study were to evaluate the immunomodulatory effects of ghrelin in male Wistar rats infected with the Y strain of T. cruzi. Methods In order to delineate an immune response against T. cruzi mediated by ghrelin, we evaluated the following parameters: quantification of blood and cardiac parasites; analysis of cell markers (CD3(+), CD8(+), NK, NKT, CD45RA(+), macrophage and RT1B(+)); nitric oxide (NO) production; lymphoproliferation assays; splenocyte apoptosis; and INF-gamma, IL-12 and IL-6 quantification in sera. Results The animals infected with T. cruzi and supplemented with ghrelin demonstrated an upregulated pattern in macrophage and NO production, whereas an anti-inflammatory response was observed in T cells and cytokines. The low response against T. cruzi mediated by T cells probably contributed to a higher colonization of the cardiac tissue, when compared to infected groups. On the other side, the peptide decreased the inflammatory infiltration in cardiac tissue infected with T. cruzi. Conclusions Ghrelin demonstrated a dual function in animals infected with T. cruzi. Further studies, especially related to the decrease of cardiac tissue inflammation, are needed in order to determine the advantages of ghrelin supplementation in Chagas disease, mostly for populations from endemic areas. (AU)

• Processo FAPESP: 14/18682-3 - Grelina: possíveis efeitos cardioprotetores e imunomoduladores na Doença de Chagas experimental aguda e crônica
• Beneficiário: Ana Amélia Carraro Abrahão
• Modalidade de apoio: Auxílio à Pesquisa - Regular