Cyclohexane-1,3-dione as a derivatizing agent for the analysis of aldehydes by micelar electrokinetic chromatography with diode array detection

Autor(es)

Donegattii, Tiago Augusto ^[1] ; Lobato, Alnilan ^[2] ; Goncalves, Luis Moreira ^[2] ; Pereira, Elisabete Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, Dept Fis Quim & Matemat, Sorocaba - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ELECTROPHORESIS; v. 40, n. 22, p. 2929-2935, NOV 2019.
• Citações Web of Science: 0

Resumo

Aldehydes are important compounds in a large number of samples, especially food and beverages. In this work, for the first time, cyclohexane-1,3-dione (CHD) was used as a derivatizing reagent aiming aldehyde (formaldehyde, acetaldehyde, propionaldehyde, and valeraldehyde) analysis by MEKC-DAD. The optimized separation of the derivates was performed using a voltage program (+20 kV, 0-15 min.; +23 kV, 15-17 min.) at a temperature of 26 degrees C, and using as the running buffer a mixture containing 100 mmol/L of sodium dodecyl sulfate and 29 mmol/L of sodium tetraborate at pH 9.2, with maximum absorbance at 260 nm. CHD was compared with two other derivatizing agents: 3-methyl-2-benzothiazolinone hydrazone and phenylhydrazine-4-sulfonic acid. The CHD-aldehyde derivatives were also characterized by LC-MS. The calibration curves for all aldehydes had r(2) above 0.999 and LODs ranged from 0.01 to 0.7 mg/L. The optimized methodology was applied in sugar cane brandy (cachaca) samples successfully. CHD showed to be an alternative derivatization reagent due to its stability, aqueous solubility, high selectivity and sensitivity, reduced impurities, and simple preparation steps. (AU)

• Processo FAPESP: 11/23286-1 - Estudos analíticos e ambientais sobre a distribuição espacial, sazonal e temporal de aldeídos de baixa massa molar presentes na atmosfera da região de Sorocaba
• Beneficiário: Elisabete Alves Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/11214-8 - Recurso à eletroforese capilar após extração por GDME-MIPs para a determinação de aldeídos
• Beneficiário: Alnilan Cristina Barros Lobato
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/14425-7 - Um novo rumo sinérgico para a microextração por difusão gasosa (GDME) fazendo uso de polímeros com impressão molecular (MIPs)
• Beneficiário: Luís Francisco Moreira Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores