Increased cytokines production and oxidative stress are related with purinergic signaling and cell survival in post-thyroidectomy hypothyroidism

Autor(es)

Baldissarelli, Jucimara ^{[1, 2]} ; Manica, Aline ^[1] ; Pillat, Micheli Mainardi ^[3] ; Bagatini, Margarete Dulce ^[4] ; Rosa Leal, Daniela Bitencourt ^[1] ; Abdalla, Fatima Husein ^{[1, 5]} ; Morsch, Vera M. ^[1] ; Ulrich, Henning ^[3] ; Bornemann, Clarissa Pereira ^[6] ; Chitolina Schetinger, Maria R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Maria, Ctr Ciencias Nat & Exatas, Programa Posgrad Ciencias Bioquim Toxicol, Campus Univ, Santa Maria, RS - Brazil

^[2] Univ Fed Pelotas, Inst Biol, Dept Fisiol & Farmacol, Campus Capao Leao, Pelotas, RS - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil

^[4] Univ Fed Fronteira Sul, Campus Chapeco, Chapeco, SC - Brazil

^[5] Univ Luterana Brasil, Unidade Univ Carazinho, Canoas, RS - Brazil

^[6] Serv Med Nucl Santa Maria CINT, Santa Maria, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 499, JAN 1 2020.
• Citações Web of Science: 0

Resumo

Thyroid hormones have essential roles in regulation of cellular functions, including the immune system. The purinergic signaling, activated through extracellular nucleotides and nucleosides has also strong implications in immune response regulation. Hypothyroidism may involve effects on the immune and purinergic systems. In view of that, we evaluated cytokines levels, their relation with the expression of purinergic enzymes and the effects of this condition on immune system cells from patients with post-thyroidectomy hypothyroidism. Increased IL6, IL10, IL17 and TNF-alpha levels as well as an increase in CD73 expression in lymphocytes were observed in patients' blood. Moreover, augmented myeloperoxidase activity, lipid peroxidation and thiolgroup production were observed in post-thyroidectomy hypothyroidism. In addition, proliferation and cell death of lymphocytes were enhanced when exposed to patients' serum. This study demonstrates that hypothyroidism is related to changes in the purinergic system, increased cytokines production and oxidative stress, which interfere in the cell life and signaling. (AU)

• Processo FAPESP: 12/50880-4 - Células-tronco: dos papéis de receptores de cininas e purinas às aplicações terapêuticas
• Beneficiário: Alexander Henning Ulrich
• Modalidade de apoio: Auxílio à Pesquisa - Temático