Regulation of murine arthritis by systemic, spinal, and intra-articular adrenoceptors

Autor(es)

Kanashiro, Alexandre ^{[1, 2]} ; de Lucena Leoncio, Thais Oliveira ^[2] ; Schneider, Ayda Henriques ^[2] ; Alves, Helio Rocha ^[2] ; Bassi, Gabriel Shimizu ^[3] ; Veloso Dutra, Sabrina Graziani ^[2] ; Cunha, Fernando de Queiroz ^[4] ; Ulloa, Luis ^[3] ; Malvar, David do Carmo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Ribeirao Preto, SP - Brazil

^[2] Univ Fed Rural Rio de Janeiro, Dept Physiol Sci, Seropedica, RJ - Brazil

^[3] Rutgers State Univ, New Jersey Med Sch, Ctr Immunol & Inflammat, Dept Surg, Newark, NJ - USA

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACOLOGICAL REPORTS; v. 71, n. 6, p. 1095-1103, DEC 2019.
• Citações Web of Science: 0

Resumo

Background: The regulation of the immune system by the sympathetic nervous system is allowing the design of novel treatments for inflammatory disorders such as arthritis. In this study, we have analyzed the effects of alpha- and beta-adrenoceptor agonists injected subcutaneously, intrathecally, or intra-articularly in zymosan-induced arthritis. Methods: Murine arthritis was induced by intra-articular (knee joint) injection of zymosan. alpha 1 (phenylephrine), alpha 2 (clonidine), beta 1 (dobutamine), or beta 2 (salbutamol)-adrenoceptor agonists were injected subcutaneously (sc), intrathecally (it), or intra-articularly (ia) to activate peripheral, spinal, or intra-articular adrenoceptors and to study their effects on articular edema formation and neutrophil migration into the synovial cavity. Results: Treatments with phenylephrine did not affect the edema formation, but it increased neutrophil migration when injected subcutaneously (155.3%) or intra-articularly (187.7%). Treatments with clonidine inhibited neutrophil migration (59.9% sc, 68.7% it, 42.8% ia) regardless of the route of administration, but it inhibited edema formation only when injected intrathecally (66.7%) or intraarticularly (36%) but not subcutaneously. Treatments with dobutamine inhibited both edema (42.0% sc, 69.5% it, 61.6% ia) and neutrophil migration (28.4% sc, 70.3% it, 82.4% ia) in a concentration dependent manner. Likewise, all the treatments with salbutamol also inhibited edema formation (89.9% sc, 62.4% it, 69.8% ia) and neutrophil migration (76.6% sc, 39.1% it, 71.7% ia). Conclusion: Whereas the beta-adrenoceptor agonists induced anti-inflammatory effects regardless of their route of administration, alpha 1- and alpha 2-adrenoceptor agonists induced either pro- and anti-inflammatory effects, respectively. (C) 2019 Maj Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/20343-4 - Via colinérgica anti-inflamatória: o papel da neuroimunomodulação no controle da resposta inflamatória
• Beneficiário: Alexandre Kanashiro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/04237-2 - Via Colinérgica Anti-inflamatória: O papel da neuroimunomodulação no controle da resposta Inflamatória.
• Beneficiário: Alexandre Kanashiro
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores