| Full text | |
| Author(s): |
Kanashiro, Alexandre
[1, 2]
;
de Lucena Leoncio, Thais Oliveira
[2]
;
Schneider, Ayda Henriques
[2]
;
Alves, Helio Rocha
[2]
;
Bassi, Gabriel Shimizu
[3]
;
Veloso Dutra, Sabrina Graziani
[2]
;
Cunha, Fernando de Queiroz
[4]
;
Ulloa, Luis
[3]
;
Malvar, David do Carmo
[2]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Ribeirao Preto, SP - Brazil
[2] Univ Fed Rural Rio de Janeiro, Dept Physiol Sci, Seropedica, RJ - Brazil
[3] Rutgers State Univ, New Jersey Med Sch, Ctr Immunol & Inflammat, Dept Surg, Newark, NJ - USA
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PHARMACOLOGICAL REPORTS; v. 71, n. 6, p. 1095-1103, DEC 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: The regulation of the immune system by the sympathetic nervous system is allowing the design of novel treatments for inflammatory disorders such as arthritis. In this study, we have analyzed the effects of alpha- and beta-adrenoceptor agonists injected subcutaneously, intrathecally, or intra-articularly in zymosan-induced arthritis. Methods: Murine arthritis was induced by intra-articular (knee joint) injection of zymosan. alpha 1 (phenylephrine), alpha 2 (clonidine), beta 1 (dobutamine), or beta 2 (salbutamol)-adrenoceptor agonists were injected subcutaneously (sc), intrathecally (it), or intra-articularly (ia) to activate peripheral, spinal, or intra-articular adrenoceptors and to study their effects on articular edema formation and neutrophil migration into the synovial cavity. Results: Treatments with phenylephrine did not affect the edema formation, but it increased neutrophil migration when injected subcutaneously (155.3%) or intra-articularly (187.7%). Treatments with clonidine inhibited neutrophil migration (59.9% sc, 68.7% it, 42.8% ia) regardless of the route of administration, but it inhibited edema formation only when injected intrathecally (66.7%) or intraarticularly (36%) but not subcutaneously. Treatments with dobutamine inhibited both edema (42.0% sc, 69.5% it, 61.6% ia) and neutrophil migration (28.4% sc, 70.3% it, 82.4% ia) in a concentration dependent manner. Likewise, all the treatments with salbutamol also inhibited edema formation (89.9% sc, 62.4% it, 69.8% ia) and neutrophil migration (76.6% sc, 39.1% it, 71.7% ia). Conclusion: Whereas the beta-adrenoceptor agonists induced anti-inflammatory effects regardless of their route of administration, alpha 1- and alpha 2-adrenoceptor agonists induced either pro- and anti-inflammatory effects, respectively. (C) 2019 Maj Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 11/20343-4 - Antiinflammatory cholinergic pathway: the role of neuroimmunomodulation in the control of inflammatory response |
| Grantee: | Alexandre Kanashiro |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/08216-2 - CRID - Center for Research in Inflammatory Diseases |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 12/04237-2 - Antiinflammatory Cholinergic Pathway: The role of neuroimmunomodulation in the control of inflammatory response. |
| Grantee: | Alexandre Kanashiro |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |