Differential sympathetic vasomotor control by spinal AT(1) and V1 a receptors in the acute phase of hemorrhagic shock

Autor(es)

Milanez, Maycon I. O. ^[1] ; Martins, Gustavo R. ^[1] ; Nishi, Erika E. ^[1] ; Bergamaschi, Cassia T. ^[1] ; Campos, Ruy R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Cardiovasc Div, Dept Physiol, Escola Paulista Med, Rua Botucatu 862, Ground Floor, BR-04023060 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmacology; v. 866, JAN 5 2020.
• Citações Web of Science: 0

Resumo

The role of the renin-angiotensin-aldosterone system and arginine vasopressin (AVP) as humoral components in maintaining blood pressure (BP) during hemorrhagic shock (HS) is well established. However, little is known about the role of angiotensin II (Ang II) and AVP in the control of preganglionic sympathetic neuron activity. We studied the effects evoked by spinal Ang II type I (AT(1)) and V1a receptors antagonism on cardiovascular and sympathetic responses during HS. A catheter (PE-10) was inserted into the subarachnoid space and advanced to the T10-11 vertebral level in urethane-anesthetized rats. The effects of HS on BP, heart rate (HR), and renal and splanchnic sympathetic nerve activity (rSNA and sSNA, respectively) were analyzed in the presence or absence (HS rats) of intrathecally injected losartan (HS-Los rats) or V1a antagonist (HS-V1a rats). The right femoral artery was catheterized for bleeding. Using a 5 ml syringe, hemorrhage was maintained continuously until a BP reduction of similar to 50 mmHg was achieved. We found that bleeding caused a reflex increase in HR, rSNA and sSNA in the HS rats. However, such responses were attenuated in the HS-Los rats. HS-V1a rats showed a reflex increase in HR, rSNA and sSNA in terms of frequency (spikes/s) but not in amplitude. Nevertheless, the BP recovery of the groups was similar. Our data showed that spinal AT(1) receptors are essential for sympathoexcitation during the acute phase of HS. Moreover, spinal AVP seems to be a neuromodulator that controls the recruitment of spinal sympathetic vasomotor neurons during the acute phase of HS. (AU)

• Processo FAPESP: 18/02671-3 - Ações funcionais e moleculares do nervo renal na doença renal crônica experimental
• Beneficiário: Cassia Marta de Toledo Bergamaschi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/01898-4 - Avaliação das projeções encéfalo-espinais no estabelecimento temporal da hiperativação simpática renal na Hipertensão Renovascular
• Beneficiário: Maycon Igor de Oliveira Milanez
• Modalidade de apoio: Bolsas no Brasil - Doutorado