The influence of female sex hormones on lung inflammation after brain death - an experimental study

Autor(es)

Bonnano Abib, Ana Luisa de Oliveira ^[1] ; Correia, Cristiano de Jesus ^[1] ; Armstrong-, Jr., Roberto ^[1] ; Ricardo-da-Silva, Fernanda Yamamoto ^[1] ; Ferreira, Sueli Gomes ^[1] ; Vidal-dos-Santos, Marina ^[1] ; Moreira, Luiz Felipe Pinho ^[1] ; Riffo-Vasquez, Yanira ^[2] ; Breithaupt-Faloppa, Ana Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Coracao InCor, Fac Med, Lab Cirurgia Cardiovasc & Fisiopatol Circulacao L, Sao Paulo, SP - Brazil

^[2] Kings Coll London, Inst Pharmaceut Sci, Sackler Inst Pulm Pharmacol, London - England

• Tipo de documento: Artigo Científico
• Fonte: Transplant International; v. 33, n. 3 NOV 2019.
• Citações Web of Science: 0

Resumo

Organ donor's age negatively influences graft survival of organs, increasing risk of complications. Aging occurs in both men and women; however, the menopause marks a decrease in sex hormones and a sudden increase in the process of vascular aging. We investigated sex hormones' influence on the lung inflammatory process induced by BD in female rats. Wistar rats were grouped as: female rats from high estradiol to heat period (non-OVx) and ovariectomized (OVx) female rats. Ovariectomy was carried out 10 days before BD. BD was induced using intracranial balloon rapid inflation. Serum hormones and inflammatory mediators were quantified, leukocytes and platelets counted and lung samples were collected for RT-PCR, immunohistochemical, and histological analysis. Female sex hormones and corticosterone were reduced 6 h after BD in non-OVx group. The infiltration of leukocytes in female non-OVx lungs was higher compared to OVx. G-CSF, VEGF, and CINC-1 were found increased in non-OVx group serum in comparison to OVx. Lung mediators were increased in non-OVx rats compared to controls. The acute reduction of sex hormones induced by BD appears to have a worse effect on lung inflammation than a reduction that has happened over a prolonged period of time, allowing a physiological adaptation prior to BD. (AU)

• Processo FAPESP: 13/20282-0 - Influência do gênero nas alterações microcirculatórias e no processo inflamatório em diferentes órgãos em modelo de morte encefálica em ratos
• Beneficiário: Ana Cristina Breithaupt Faloppa
• Modalidade de apoio: Auxílio à Pesquisa - Regular