Evaluation of Hepatic Steatosis in Rodents by Time-Domain Nuclear Magnetic Resonance

Autor(es)

Pedroso, Joao A. B. ^[1] ; Camporez, Joao Paulo ^[1] ; Belpiede, Luciana T. ^[1] ; Pinto, Rafaela S. ^[1] ; Cipolla-Neto, Jose ^[1] ; Donato, Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTICS; v. 9, n. 4 DEC 2019.
• Citações Web of Science: 0

Resumo

Devices that analyze body composition of rodents by time-domain nuclear magnetic resonance (TD-NMR) are becoming popular in research centers that study metabolism. Theoretically, TD-NMR devices can also evaluate lipid content in isolated tissues. However, the accuracy of TD-NMR to determine hepatic steatosis in the liver of small laboratory animals has not been evaluated in detail. We observed that TD-NMR was able to detect increased lipid content in the liver of rats consuming high-fat diet (HFD) for 12 weeks and in genetically obese (Lep(ob/ob) and Lepr(db/db)) mice. The lipid content determined by TD-NMR showed a positive correlation with triglyceride content measured by colorimetric assays. In contrast, TD-NMR did not detect hepatic steatosis in C57BL/6 mice consuming HFD for 4 or 12 weeks, despite their obesity and increased liver triglyceride content. These findings indicate that tissue mass and the severity of hepatic steatosis affect the sensitivity of TD-NMR to detect liver lipid content. (AU)

• Processo FAPESP: 14/50457-0 - Melatonina e a regulação do metabolismo energético: estudos básicos, clínicos e epidemiológicos
• Beneficiário: José Cipolla Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/04956-5 - Impacto do receptor de estrogênio alpha na Doença Hepática Gordurosa não Alcoólica e metabolismo energético do fígado
• Beneficiário: João Paulo Gabriel Camporez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/02983-2 - Ação do hormônio do crescimento no sistema nervoso: relevância para as funções neurais e na doença
• Beneficiário: Jose Donato Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático