Mygalin: An Acylpolyamine With Bactericidal Activity

Autor(es)

Espinoza-Culupu, Abraham ^{[1, 2]} ; Mendes, Elizabeth ^[1] ; Vitorino, Hector Aguilar ^[3] ; da Silva Jr, Pedro Ismael ; Borges, Monamaris Marques ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Bacteriol Lab, Sao Paulo - Brazil

^[2] Univ Sao Paulo, PhD Program Biotechnol, Sao Paulo - Brazil

^[3] Univ Maryland, Columbus Ctr, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 - USA

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 10, JAN 10 2020.
• Citações Web of Science: 0

Resumo

Inappropriate use of antibiotics favors the selection and spread of resistant bacteria. To reduce the spread of these bacteria, finding new molecules with activity is urgent and necessary. Several polyamine analogs have been constructed and used to control microorganisms and tumor cells. Mygalin is a synthetic acylpolyamine, which are analogs of spermidine, derived from the hemolymph of the spider Acanthoscurria gomesiana. The effective activity of polyamines and their analogs has been associated with their structure. The presence of two acyl groups in the Mygalin structure may give this molecule a specific antibacterial activity. The aim of this study was to identify the mechanisms involved in the interaction of Mygalin with Escherichia coli to clarify its antimicrobial action. The results indicated that Mygalin exhibits intense dose and time-dependent bactericidal activity. Treatment of E. coli with this molecule caused membrane rupture, inhibition of DNA synthesis, DNA damage, and morphological changes. The esterase activity increased along with the intracellular production of reactive oxygen species (ROS) after treatment of the bacteria with Mygalin. In addition, this molecule was able to sequester iron and bind to LPS. We have shown that Mygalin has bactericidal activity with underlying mechanisms involving ROS generation and chelation of iron ions that are necessary for bacterial metabolism, which may contribute to its microbicidal activity. Taken together, our data suggest that Mygalin can be explored as a new alternative drug with antimicrobial potential against Gram-negative bacteria or other infectious agents. (AU)

• Processo FAPESP: 14/04307-6 - Migalina: mecanismo imunomodulador e seu potencial no controle de infecções.
• Beneficiário: Bruna de Sousa Melo
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 13/11212-9 - Migalina: mecanismo imunomodulador e seu potencial no controle de infecções bacterianas
• Beneficiário: Monamaris Marques Borges
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs