Genome-wide association study: Understanding the genetic basis of the gait type in Brazilian Mangalarga Marchador horses, a preliminary study

Autor(es):Mostrar menos -

Bussiman, Fernando de Oliveira ^[1] ; dos Santos, Bruna Aparecida ^[2] ; Abreu Silva, Barbara da Conceicao ^[3] ; Mamani Mamani, Gerardo Cornelio ^[3] ; Grigoletto, Lais ^[3] ; Pereira, Guilherme Luis ^[2] ; Ferraz, Guilherme de Camargo ^[4] ; Sterman Ferraz, Jose Bento ^[3] ; Mattos, Elisangela Chicaroni ^[3] ; Eler, Joanir Pereira ^[3] ; Ventura, Ricardo Vieira ^[5] ; Curi, Rogerio Abdallah ^[2] ; de Carvalho Balieiro, Julio Cesar ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Anim Sci, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Sao Paulo State Univ FMVZ UNESP, Coll Vet Med & Anim Sci, Dept Anim Improvement & Nutr, Rua Jose Barbosa de Barros 1780, BR-18618307 Botucatu, SP - Brazil

^[3] Univ Sao Paulo GMAB FZEA USP, Grp Anim Breeding & Biotechnol, Dept Vet Sci, Coll Anim Sci & Food Engn, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[4] Sao Paulo State Univ FCAV UNESP, Coll Agr & Vet Sci, Dept Morphol & Anim Physiol, Access Route Paulo Donato Castellane, BR-14884900 Jaboticabal, SP - Brazil

^[5] Univ Sao Paulo FMVZ USP, Coll Vet Med & Anim Sci, Dept Anim Nutr & Prod, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIVESTOCK SCIENCE; v. 231, JAN 2020.
• Citações Web of Science: 2

Resumo

The ECA23:22999655C > A DMRT3 variant has been described as a causative region for the four-beat gait in horses, however it remains uncertain for the two distinct gait types observed in the Brazilian Mangalarga Marchador (MM) breed. This study aimed to perform a single-step GWAS, prospecting the genetic architecture of the marcha batida (MB) and marcha picada (MP) in MM horses. Phenotypic records from 1,230 animals in a pedigree file containing 3172 individuals were considered in the current analysis. A total of 192 (86 MB and 106 MP) horses were genotyped with the 670 k equine genotyping array (Thermo Fisher Scientific), and 48 horses were imputed from 65k (Illumina) to 670k. The GWAS was conducted in a Bayesian approach. For the estimation of SNP effects, three iterations were performed. The position from SNPs that explained more than 1% of the additive genetic variance were used to prospect genomic regions in windows of 250 kb. From the 69 identified genes, 39 were not annotated in horses and were blasted against the NCBI database. The enrichment analysis revealed that eight metabolic pathways might be involved with the gait type since most were related to the metabolism of amino acid, energy, and immune system. The found genes were associated with differences in the energetic metabolism between animals from the marcha batida or marcha picada groups, as well as with metabolic changes after gait exercise. (AU)

• Processo FAPESP: 16/17834-0 - Estudo de associação genômica ampla para as diferenças genéticas entre as marchas batida e picada em equinos Mangalarga Marchador
• Beneficiário: Fernando de Oliveira Bussiman
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/19081-9 - Prospecção de regiões cromossômicas, genes e SNP relacionados à marcha Batida e análise genômica da estrutura populacional em cavalos da raça brasileira Mangalarga Marchador
• Beneficiário: Rogério Abdallah Curi
• Modalidade de apoio: Auxílio à Pesquisa - Regular