Serum IgG Anti-Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

Autor(es)

Nakashima, Fabiana ^[1] ; Pardo, Valquiria Sousa ^[1] ; Miola, Marcos Paulo ^{[1, 2]} ; Antunes Murata, Fernando Henrique ^[3] ; Paduan, Natalia ^[1] ; Longo, Stefani Miqueline ^[1] ; Brandao de Mattos, Cinara Cassia ^{[1, 3]} ; Pereira-Chioccola, Vera Lucia ^[4] ; Ricci, Octavio ^[2] ; de Mattos, Luiz Carlos ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose Rio Preto, Mol Biol Dept, Immunogenet Lab, Sao Paulo - Brazil

^[2] Fundacao Fac Reg Med, Blood Bank Sao Jose Rio Preto, Sao Paulo - Brazil

^[3] FAMERP Toxoplasma Res Grp, Sao Paulo - Brazil

^[4] Adolfo Lutz Inst, Parasite Mol Biol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 9, JAN 14 2020.
• Citações Web of Science: 0

Resumo

Background: Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-T. gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t-test or Mann-Whitney according to the normal distribution (p-value <= 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM(+)/IgG(-): 5 (0.6%); IgM(+)/IgG(+): 21 (2.8%); IgM(-)/IgG(+): 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions: In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors. (AU)

• Processo FAPESP: 12/07716-9 - Investigação sorológica e molecular de Toxoplasma gondii em doadores de sangue
• Beneficiário: Luiz Carlos de Mattos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/07750-2 - Investigação sorológica e molecular de Toxoplasma gondii em doadores de sangue
• Beneficiário: Fabiana Nakashima
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/15879-8 - Infecção por Toxoplasma gondii: caracterização sorológica e molecular em pacientes atendidos pelo SUS
• Beneficiário: Fernando Henrique Antunes Murata
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/00900-4 - Investigação sorológica e molecular de Toxoplasma gondii em doadores de sangue
• Beneficiário: Valquiria Pardo de Sousa
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 11/13939-8 - Estudo de um grupo de infecções fúngico-parasitárias visando a melhoria das técnicas de caracterização e diagnóstico imuno-molecular
• Beneficiário: Vera Lúcia Pereira Chioccola
• Modalidade de apoio: Auxílio à Pesquisa - Regular