| Full text | |
| Author(s): |
Nakashima, Fabiana
[1]
;
Pardo, Valquiria Sousa
[1]
;
Miola, Marcos Paulo
[1, 2]
;
Antunes Murata, Fernando Henrique
[3]
;
Paduan, Natalia
[1]
;
Longo, Stefani Miqueline
[1]
;
Brandao de Mattos, Cinara Cassia
[1, 3]
;
Pereira-Chioccola, Vera Lucia
[4]
;
Ricci, Octavio
[2]
;
de Mattos, Luiz Carlos
[1, 3]
Total Authors: 10
|
| Affiliation: | [1] Fac Med Sao Jose Rio Preto, Mol Biol Dept, Immunogenet Lab, Sao Paulo - Brazil
[2] Fundacao Fac Reg Med, Blood Bank Sao Jose Rio Preto, Sao Paulo - Brazil
[3] FAMERP Toxoplasma Res Grp, Sao Paulo - Brazil
[4] Adolfo Lutz Inst, Parasite Mol Biol Lab, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 9, JAN 14 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-T. gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t-test or Mann-Whitney according to the normal distribution (p-value <= 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM(+)/IgG(-): 5 (0.6%); IgM(+)/IgG(+): 21 (2.8%); IgM(-)/IgG(+): 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions: In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors. (AU) | |
| FAPESP's process: | 12/07716-9 - Serological and molecular investigation of Toxoplasma gondii in blood donors |
| Grantee: | Luiz Carlos de Mattos |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/07750-2 - Serological and molecular investigation of Toxoplasma gondii in blood donors |
| Grantee: | Fabiana Nakashima |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS) |
| Grantee: | Fernando Henrique Antunes Murata |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/13939-8 - Study of a group of fungal and parasitic infections aiming the improvement of techniques of characterization and immune-molecular diagnosis |
| Grantee: | Vera Lúcia Pereira Chioccola |
| Support Opportunities: | Regular Research Grants |