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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serum IgG Anti-Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

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Author(s):
Nakashima, Fabiana [1] ; Pardo, Valquiria Sousa [1] ; Miola, Marcos Paulo [1, 2] ; Antunes Murata, Fernando Henrique [3] ; Paduan, Natalia [1] ; Longo, Stefani Miqueline [1] ; Brandao de Mattos, Cinara Cassia [1, 3] ; Pereira-Chioccola, Vera Lucia [4] ; Ricci, Octavio [2] ; de Mattos, Luiz Carlos [1, 3]
Total Authors: 10
Affiliation:
[1] Fac Med Sao Jose Rio Preto, Mol Biol Dept, Immunogenet Lab, Sao Paulo - Brazil
[2] Fundacao Fac Reg Med, Blood Bank Sao Jose Rio Preto, Sao Paulo - Brazil
[3] FAMERP Toxoplasma Res Grp, Sao Paulo - Brazil
[4] Adolfo Lutz Inst, Parasite Mol Biol Lab, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 9, JAN 14 2020.
Web of Science Citations: 0
Abstract

Background: Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-T. gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t-test or Mann-Whitney according to the normal distribution (p-value <= 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM(+)/IgG(-): 5 (0.6%); IgM(+)/IgG(+): 21 (2.8%); IgM(-)/IgG(+): 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions: In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors. (AU)

FAPESP's process: 12/07716-9 - Serological and molecular investigation of Toxoplasma gondii in blood donors
Grantee:Luiz Carlos de Mattos
Support type: Regular Research Grants
FAPESP's process: 12/07750-2 - Serological and molecular investigation of Toxoplasma gondii in blood donors
Grantee:Fabiana Nakashima
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)
Grantee:Fernando Henrique Antunes Murata
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/13939-8 - Study of a group of fungal and parasitic infections aiming the improvement of techniques of characterization and immune-molecular diagnosis
Grantee:Vera Lúcia Pereira Chioccola
Support type: Regular Research Grants