Fungal biocatalysts for labdane diterpene hydroxylation

Autor(es)

Cruz de Carvalho, Tatiane ^[1] ; de Oliveira Silva, Eliane ^{[1, 2]} ; Soares, Gilberto Augusto ^[3] ; Parreira, Renato Luis Tame ^[3] ; Ambrosio, Sergio Ricardo ^[3] ; Jacometti Cardoso Furtado, Niege Aracari ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Bahia, Inst Chem, Dept Organ Chem, Rua Barao de Jeremoabo S-N, BR-40110060 Salvador, BA - Brazil

^[3] Univ Franca, Nucleus Res Sci & Technol, Franca, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 43, n. 6 FEB 2020.
• Citações Web of Science: 0

Resumo

Labdane diterpenes and their derivatives have shown remarkable biological activities and are useful as chiral building blocks for the synthesis of a variety of bioactive compounds. There is great interest in developing biocatalyst technology to achieve regio- and stereoselective hydroxylation of unactivated C-H bonds in complex natural products, since the functionalization of unactivated C-H bonds generally requires hard reaction conditions and highly reactive oxidizing agents, which are limited regarding the control of regio- and stereoselectivity. Filamentous fungi are efficient biocatalysts capable of catalyzing a wide variety of hydroxylation reactions, and the use of whole cell biocatalysts provides advantages regarding cofactor regeneration and is much less expensive. Therefore, the goal of this study was to select biocatalysts to develop biotransformation processes that can be scalable under mild reaction conditions for hydroxylation of a labdane diterpene, 3 beta-acetoxy-copalic acid, which contains the trans-decalin moiety and a side chain dienic system appropriate for the preparation of a variety of compounds. Biotransformation processes were carried out and five filamentous fungi were selected as capable of producing hydroxylated diterpenes at positions C-3, C-6, C-7 and C-18 of the trans-decalin moiety and C-13 of the side chain dienic system. Hydroxylation reactions occurred with regio- and stereoselectivity by using some fungi that produced only the 6 alpha, 7 alpha and 13 alpha-hydroxyl derivatives. The chemical structures of the hydroxylated diterpenes were determined from spectrometric and spectroscopic data, and the relative stereochemistry of stereogenic centers was established from coupling constants, by NOE-diff experiments and/or by computational calculations. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/25201-7 - Estudos de bioacessibilidade, transporte intestinal e biotransformação in vitro dos ácidos ent-hardwickiico e ent-poliáltico das oleorresinas de Copaifera pubiflora e Copaifera duckei
• Beneficiário: Niege Araçari Jacometti Cardoso Furtado
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/01766-4 - Biotransformações do ²-cariofileno e do ácido 3-acetoxi-copálico: utilização de fungos filamentosos e bactérias do trato gastrointestinal para estudos de metabolismo in vitro e avaliação da atividade citotóxica dos derivados obtidos.
• Beneficiário: Tatiane Cruz de Carvalho
• Linha de fomento: Bolsas no Brasil - Doutorado