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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Synthesis and structural characterization of a series of ternary copper(II)-L-dipeptide-neocuproine complexes. Study of their cytotoxicity against cancer cells including MDA-MB-231, triple negative breast cancer cells

Texto completo
Autor(es):
Alvarez, Natalia [1] ; Vina, Diana [1] ; Leite, Celisnolia M. [2] ; Mendes, Luis F. S. [3] ; Batista, Alzir A. [2] ; Ellena, Javier [4] ; Costa-Filho, Antonio J. [3] ; Facchin, Gianella [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Republica, Fac Quim, Av Gen Flores 2124, Montevideo - Uruguay
[2] Univ Fed Sao Carlos, Dept Quim, CP 676, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Av Bandeirantes, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Inst Fis Sao Carlos, CP 369, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Inorganic Biochemistry; v. 203, FEB 2020.
Citações Web of Science: 0
Resumo

This work presents the synthesis and characterization of eight copper complexes {[}Cu(L-dipeptide)(neo)]center dot nH(2)O (neo = neocuproine) and their cytotoxic activities against tumor cell lines. The crystalline structure of {[}Cu(gly-val)(neo)]center dot 3H(2)O, {[}Cu(gly-leu)(neo)]center dot H2O, {[}Cu(ala-gly)(neo)]center dot 4H(2)O, {[}Cu(val-phe)(neo)]center dot 4.5H(2)O and {[}Cu(phephe)(neo)]center dot 3H(2)O were determined by single crystal X-ray diffraction. In all of them, the Cu(II) is pentacoordinated, in a square pyramidal environment. The coordination observed in solid state was retained in the major species in aqueous solution, as suggested by Electronic Paramagnet Resonance and UV-vis spectroscopies. The complexes were shown to have affinity for isolated DNA, as determined by Circular Dichroism experiments. Furthermore, biological experiments showed that all the complexes present high cytotoxic activity against the cell lines: MDA-MB-231, MCF-7 (human metastatic breast adenocarcinomas, the first triple negative), MCF-10A (human normal breast cells), A549 (human lung epithelial carcinoma) and MRC-5 (human lung epithelial cells). Together, these results suggest that these compounds are promising steps towards new effective drugs to treat cancer. (AU)

Processo FAPESP: 15/50366-7 - Resolving mechanistic details of peptide transport across membranes using crystallographic and non-crystallographic structural biology approaches
Beneficiário:Antonio José da Costa Filho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/24669-8 - Desvendando as bases moleculares de processos iniciais da secreção de proteínas em humanos utilizando técnicas biofísicas
Beneficiário:Luis Felipe Santos Mendes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado