Shifts in the bacterial community of saliva give insights on the relationship between obesity and oral microbiota in adolescents

Autor(es)

de Andrade, Pedro Avelino Maia ^{[1, 2]} ; Giovani, Priscila Alves ^[3] ; Araujo, Darlle Santos ^[3] ; de Souza, Adijailton Jose ^[1] ; Pedroni-Pereira, Aline ^[3] ; Kantovitz, Kamila Rosamilia ^{[3, 4]} ; Andreote, Fernando Dini ^[1] ; Castelo, Paula Midori ^[5] ; Nociti-Jr, Francisco Humberto

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo Esalq USP, Dept Soil Sci, Coll Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo Esalq USP, Dept Genet, Coll Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Piracicaba, SP - Brazil

^[4] Sao Leopoldo Mandic Res Ctr, Dept Dent Mat, Campinas, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Biol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Microbiology; v. 202, n. 5 FEB 2020.
• Citações Web of Science: 0

Resumo

The current study aimed at the determination of the impact of obesity on the salivary microbiome in adolescents. Sixty subjects ranging 14-17 years old were enrolled (obese: n = 30-50% females, and normal weight: n = 30-50% females). Stimulated saliva was collected for denaturing gradient gel electrophoresis (DGGE) band patterns and massive 16S rRNA gene sequencing using the Ion Torrent platform. Overall, data analysis revealed that male subjects harbored a higher diverse salivary microbiome, defined by a significant higher richness (32.48 versus 26.74) and diversity (3.36 versus 3.20), higher Simpson values (0.96 versus 0.95) and distinct bacterial community structure considering either sex or condition (p < 0.05). Bacterial community fingerprinting analysis in human saliva showed a positive correlation with increased body mass index (BMI) in adolescents. Veillonella, Haemophilus and Prevotella occurrence was found to be affected by BMI, whereas Neisseria and Rothia occurrence was significantly impacted by sex in obese subjects. Our findings suggest that male and female adolescents may harbor a naturally distinct salivary microbiota and that obesity may specifically have an impact on their oral bacterial community. The potential dysbiotic oral microbiome in obese adolescents raises new insights on the etiology and prevention of future conditions in these populations. (AU)

• Processo FAPESP: 16/02942-1 - Análise Global do Protema do Ligamento Periodontal.
• Beneficiário: Priscila Alves Giovani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/24804-4 - Avaliação da composição salivar e da qualidade da função mastigatória de adolescentes com sobrepeso e obesidade
• Beneficiário: Paula Midori Castelo Ferrua
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/04492-2 - Avaliação nutricional, salivar, mastigatória e da qualidade de vida de crianças pré-púberes com sobrepeso e obesas
• Beneficiário: Paula Midori Castelo Ferrua
• Modalidade de apoio: Auxílio à Pesquisa - Regular