Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli

Autor(es)

Rodrigues, Juliana Falcao ^{[1, 2]} ; Lourenco, Rogerio Ferreira ^{[3, 2]} ; Maeda, Denicar Lina Nascimento Fabris ^{[1, 2, 4]} ; de Jesus Cintra, Mariana ^[2] ; Nakao, Naomi ^[2] ; Mathias-Santos, Camila ^{[2, 5]} ; Luiz, Wilson Barros ^{[6, 2]} ; de Souza Ferreira, Luis Carlos ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Vaccine Dev Lab, Dept Microbiol, Inst Biomed Sci, Ave Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Sao Paulo - Brazil

^[4] Univ Virginia, Dept Pediat, Charlottesville, VA - USA

^[5] Criminalist Inst, Tech Sci Police Superintendency, Sao Paulo, SP - Brazil

^[6] Univ Estadual Santa Cruz, Dept Biol Sci, Ilheus, BA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 51, n. 2 FEB 2020.
• Citações Web of Science: 0

Resumo

Enterotoxigenic Escherichia coli (ETEC) represents one of the most important etiological agents of diarrhea in developing countries and characteristically produces at least one of two enterotoxins: heat-labile toxin (LT) and heat-stable toxin (ST). It has been previously shown that the production and release of LT by human-derived ETEC strains are variable. Although the natural genetic polymorphisms of regulatory sequences of LT-encoding (eltAB) genes may explain the variable production of LT, the knowledge of the transcriptional and posttranscriptional aspects affecting LT expression among ETEC strains is not clear. To further understand the factors affecting LT expression, we evaluated the impact of the natural polymorphism in noncoding regulatory sequences of eltAB among clinically derived ETEC strains. Sequence analyses of seven clinically derived strains and the reference strain H10407 revealed polymorphic sites at both the promoter and upstream regions of the eltAB operon. Operon fusion assays with GFP revealed that specific nucleotide changes in the Pribnow box reduce eltAB transcription. Nonetheless, the total amounts of LT produced by the tested ETEC strains did not strictly correspond to the detected LT-specific mRNA levels. Indeed, the stability of LT varied according to the tested strain, indicating the presence of posttranscriptional mechanisms affecting LT expression. Taken together, our results indicate that the production of LT is a strain-specific process and involves transcriptional and posttranscriptional mechanisms that regulate the final amount of toxin produced and released by specific strains. (AU)

• Processo FAPESP: 04/02242-2 - Diversidade genética do óperon etx em Amostras de Escherichia coli enterotoxigênica (ETEC): polimorfismo de nucleotídeos dos genes estruturais e produção da toxina LT
• Beneficiário: Juliana Falcao Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/20045-7 - Descoberta de antígenos e desenvolvimento de métodos de diagnóstico sorológico e estratégias vacinais contra o Vírus Zika (ZIKV)
• Beneficiário: Luis Carlos de Souza Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático