First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Autor(es)

Paziani, Mario Henrique ^{[1, 2]} ; Carvalho, Ludmilla Tonani ^[1] ; Carvalho Melhem, Marcia de Souza ^{[3, 4]} ; Gottardo de Almeida, Margarete Teresa ^[5] ; Nadaletto Bonifacio da Silva, Maria Emilia ^[1] ; Martinez, Roberto ^[6] ; Santos, Cledir ^[2] ; von Zeska Kress, Marcia Regina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040900 Ribeirao Preto, SP - Brazil

^[2] Univ La Frontera, Dept Chem Sci & Nat Resources, BIOREN UFRO, Temuco 4811230 - Chile

^[3] Univ Fed Mato Grosso do Sul, Fac Med, BR-79070900 Campo Grande, MS - Brazil

^[4] Govt Sao Paulo State, Secretary Hlth, BR-01246000 Sao Paulo, SP - Brazil

^[5] Fac Med Sao Jose do Rio Preto FAMERP, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[6] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14040900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 8, n. 1 JAN 2020.
• Citações Web of Science: 0

Resumo

The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1 alpha) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil. (AU)

• Processo FAPESP: 17/25300-8 - O estudo de isolados clínicos fúngicos de diferentes sítios anatômicos e a metagenômica do microbioma do pulmão de pacientes com fibrose cística
• Beneficiário: Marcia Regina von Zeska Kress
• Modalidade de apoio: Auxílio à Pesquisa - Regular