Detection of Zika virus in paired urine and amniotic fluid samples from symptomatic and asymptomatic women and their babies during a disease outbreak: association with neurological symptoms in newborns

Autor(es):Mostrar menos -

Vedovello, Danila ^[1] ; Witkin, Steven S. ^{[2, 3]} ; Botelho Silva, Andrea Cristina ^[1] ; Gillo Fajardo, Thamirys Cosmo ^[1] ; Mello, Alexandra Siqueira ^[1] ; Antunes Pascalicchio Bertozzi, Ana Paula ^{[1, 2]} ; da Silva, Alify Bertoldo ^[2] ; Vieira Filho, Nemesio Florence ^[2] ; Duarte Rodrigues, Maria Manoela ^[2] ; Gazeta, Rosa Estela ^[2] ; Moron, Antonio Fernandes ^[4] ; Pereira Sarmento, Stephanno Gomes ^[5] ; Soriano-Arandes, Antonio ^[6] ; Passos, Saulo Duarte ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Jundiai Sch Med, Lab Pediat Infectol, Rua Francisco Telles 250, BR-13202550 Jundiai, SP - Brazil

^[2] Univ Hosp, Jundiai Sch Med, Jundiai, SP - Brazil

^[3] Weill Cornell Med, New York, NY - USA

^[4] Paulista Fed Med Sch, Neonatal Perinatal Med Forum, Sao Paulo, SP - Brazil

^[5] Paulista Fed Med Sch, Paulista Ctr Fetal Med, Sao Paulo, SP - Brazil

^[6] Hosp Univ Vall dHebron, Pediat Infect Dis Unit, Barcelona - Spain

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEUROVIROLOGY; v. 26, n. 1, p. 70-76, FEB 2020.
• Citações Web of Science: 0

Resumo

Paired maternal and newborn urine and amniotic fluid from 138 subjects collected during a Zika virus (ZIKV) outbreak was analyzed for ZIKV by gene amplification (RT-qPCR), and the findings were correlated with clinical symptoms and neurological anomalies in the babies. ZIKV was detected in 1 of 9 symptomatic women (11.1%) and in 19 of 129 asymptomatic women (14.7%). Neurological manifestations were present in 19 babies (13.7%), 10 of 20 (50%) positive and 9 of 119 (7.6%) negative (p < 0.001) for ZIKV. Twelve (8.6%) urines collected during gestation were ZIKV-positive; only 2 remained positive for ZIKV postpartum. Six (4.1%) newborn urines collected within 1 day of delivery were ZIKV-positive cases. In 3 of these cases, ZIKV was detected in mother's urine pre- and postpartum and in both mother's urine and babies' urine. Four of the amniotic fluid samples (2.9%) were ZIKV-positive. Among ZIKV-negative babies with neurological sequel, 87.5% were female; in contrast, 72.7% ZIKV-positive babies with neurological abnormalities were male (p = 0.019). We conclude that during a ZIKV outbreak, clinical symptoms and ZIKV detection in biological fluids are poor predictors of infection and adverse neurologic sequel in newborns. (AU)

• Processo FAPESP: 16/08578-0 - Infecção vertical pelo vírus Zika e suas repercussões na área materno-infantil
• Beneficiário: Saulo Duarte Passos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/22007-8 - Infecção vertical pelo vírus Zika e suas repercussões na área materno-infantil
• Beneficiário: Danila Vedovello de Jesus
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado