Prostaglandin E-2 Affects Interleukin 6 and Monocyte Chemoattractant Protein 1/CCL2 Production by Cultured Stem Cells of Apical Papilla

Autor(es)

Bosio Meneses, Claudia Caroline ^[1] ; Pizzatto, Lais Nicolay ^[1] ; Fares Andrade, Fernanda Fachini ^[1] ; Sipert, Carla Renata ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Av Prof Lineu Prestes, 2227, Cidade Univ, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 46, n. 3, p. 413-418, MAR 2020.
• Citações Web of Science: 1

Resumo

Introduction: Root canal treatment of immature necrotic teeth is a major challenge in current endodontics. The effect of inflammatory mediators, such as prostaglandin, on the modulation of stem cells of the apical papilla (SOAP) is not completely understood. The aim of this study was to investigate the role of prostaglandin E-2 (PGE(2)) on SOAP activation by Escherichia coli lipopolysaccharide (LPS) in vitro. Methods: SOAP cultures were established and characterized. Increasing concentrations of lipopolysaccharide (0.1-10 mu g/mL) were used to investigate cyclooxygenase-2 (COX-2/PTGS2) and PGE(2) receptors (EP1-4) gene expression. Then, SOAP were treated with a COX-2 inhibitor (indomethacin) before treatment with different concentrations of LPS. The levels of the chemokine CCL2/monocyte chemo-attractant protein 1 and interleukin (IL)-6 were detected in cell supernatants (24 hours) by enzyme-linked immunosorbent assay. Data analysis was performed using analysis of variance followed by the Tukey post test. Results: The expression of COX-2 was up-regulated in the group treated with LPS at 1 mu g/mL compared with that in the control group. EP1-4 were detected in all experimental conditions at similar levels. SOAP treated with indomethacin presented a down-regulation in the production of LPS-induced CCL2 and the secretion of IL-6. Conclusions: SOAP showed increased COX-2 (PTGS2) gene expression induced by LPS and a PGE2-dependent production of IL-6 and CCL2. (AU)

• Processo FAPESP: 16/13944-5 - Papel de mediadores inflamatórios na modulação de células de papila apical humana in vitro
• Beneficiário: Carla Renata Sipert
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/16473-3 - Estudo do papel de angiotensina II na diferenciação de células de papila apical humana in vitro
• Beneficiário: Laís Nicolay Pizzatto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/01737-8 - Papel de endocanabinóides na modulação de células de papila apical humana in vitro
• Beneficiário: Claudia Caroline Bosio Meneses
• Modalidade de apoio: Bolsas no Brasil - Doutorado