The roles of triiodothyronine and irisin in improving the lipid profile and directing the browning of human adipose subcutaneous cells

Autor(es)

de Oliveira, Miriane ^[1] ; Mathias, Lucas Solla ^[1] ; Rodrigues, Bruna Moretto ^[1] ; Mariani, Bianca Goncalves ^[1] ; Graceli, Jones Bernardes ^[2] ; De Sibio, Maria Teresa ^[1] ; Castro Olimpio, Regiane Marques ^[1] ; Fontes Moretto, Fernanda Cristina ^[1] ; Depra, Igor Carvalho ^[1] ; Nogueira, Celia Regina ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Clin, Botucatu, SP - Brazil

^[2] Univ Fed Espirito Santo, Dept Morphol, Vitoria, ES - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 506, APR 15 2020.
• Citações Web of Science: 0

Resumo

Triiodothyronine (T3) and irisin (I) can modulate metabolic status, increase heat production, and promote differentiation of white adipose tissue (WAT) into brown adipose tissue (BAT). Herein, human subcutaneous white adipocytes were treated with 10 nM T3 or 20 nM I for 24 h to evaluate intracellular lipid accumulation, triglyceride, and glycerol levels, oxidative stress, DNA damage, and protein levels of uncoupling protein 1 (UCP1), adiponectin, leptin, peroxisome proliferator-activated receptor gamma (PPAR gamma), and fibronectin type III domain-containing protein 5 (FNDC5). T3 and irisin improved UCP1 production, lipid profile, oxidative stress, and DNA damage. T3 elevated adiponectin and leptin levels with a concomitant decrease in PPARy and FNDC5 levels. However, irisin did not alter adipokine, PPARy, and FNDC5 levels. The results indicate that T3 may be used to increase leptin and adiponectin levels to improve insulin sensitivity, and irisin may be used to prevent obesity or maintain weight due to its impact on the lipid profile without altering adipokine levels. (AU)

• Processo FAPESP: 16/03242-3 - Análise global do transcriptoma de células adiposas subcutâneas primárias humanas: identificação de genes regulados por triiodotironina e irisina
• Beneficiário: Miriane de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado