The roles of triiodothyronine and irisin in improving the lipid profile and directing the browning of human adipose subcutaneous cells

Author(s)

de Oliveira, Miriane ^[1] ; Mathias, Lucas Solla ^[1] ; Rodrigues, Bruna Moretto ^[1] ; Mariani, Bianca Goncalves ^[1] ; Graceli, Jones Bernardes ^[2] ; De Sibio, Maria Teresa ^[1] ; Castro Olimpio, Regiane Marques ^[1] ; Fontes Moretto, Fernanda Cristina ^[1] ; Depra, Igor Carvalho ^[1] ; Nogueira, Celia Regina ^[1]

Affiliation

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Clin, Botucatu, SP - Brazil

^[2] Univ Fed Espirito Santo, Dept Morphol, Vitoria, ES - Brazil

• Document type: Journal article
• Source: Molecular and Cellular Endocrinology; v. 506, APR 15 2020.
• Web of Science Citations: 0

Abstract

Triiodothyronine (T3) and irisin (I) can modulate metabolic status, increase heat production, and promote differentiation of white adipose tissue (WAT) into brown adipose tissue (BAT). Herein, human subcutaneous white adipocytes were treated with 10 nM T3 or 20 nM I for 24 h to evaluate intracellular lipid accumulation, triglyceride, and glycerol levels, oxidative stress, DNA damage, and protein levels of uncoupling protein 1 (UCP1), adiponectin, leptin, peroxisome proliferator-activated receptor gamma (PPAR gamma), and fibronectin type III domain-containing protein 5 (FNDC5). T3 and irisin improved UCP1 production, lipid profile, oxidative stress, and DNA damage. T3 elevated adiponectin and leptin levels with a concomitant decrease in PPARy and FNDC5 levels. However, irisin did not alter adipokine, PPARy, and FNDC5 levels. The results indicate that T3 may be used to increase leptin and adiponectin levels to improve insulin sensitivity, and irisin may be used to prevent obesity or maintain weight due to its impact on the lipid profile without altering adipokine levels. (AU)

• FAPESP's process: 16/03242-3 - Global transcriptome analysis of primary subcutaneous preadipocytes~ normal, human: identification of genes regulated by triiodothyronine and irisin
• Grantee: Miriane de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral