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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from Crotalus durissus terrificus by Hispidulin from Moquiniastrum floribundum

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Autor(es):
dos Santos Junior, Adeilso Bispo [1] ; Tamayose, Cinthia I. [2] ; Ferreira, Marcelo J. P. [2] ; Belchor, Mariana N. [1] ; Costa, Caroline R. C. [1] ; de Oliveira, Marcos Antonio [3] ; Toyama, Marcos Hikari [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias, BIOMOLPEP Grp, Campus Litoral Paulista, BR-11380972 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo 05508090 - Brazil
[3] UNESP, Inst Biociencias, LABIMES, Campus Litoral Paulista, BR-11380972 Sao Vicente, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Molecules; v. 25, n. 2 JAN 2 2020.
Citações Web of Science: 0
Resumo

Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on Crotalus durissus terrificus sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the Moquiniastrum floribundum leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from Crotalus durissus terrificus. (AU)

Processo FAPESP: 17/20291-0 - Caracterização da atividade pró-inflamatória de uma nova serino protease (cdtsp-2) purificada do veneno total de Crotalus durissus terrificus
Beneficiário:Marcos Hikari Toyama
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/21593-2 - Baccharis L. (Asteraceae) e fungos endofíticos associados: metabólitos secundários e atividades biológicas
Beneficiário:Marcelo José Pena Ferreira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/19942-7 - Busca de inibidores do sistema peroxirredoxina de patógenos e humanos
Beneficiário:Marcos Antonio de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/20932-8 - Estudo fitoquímico, quimiossistemático e atividades biológicas de espécies de Moquiniastrum e Richterago
Beneficiário:Cinthia Indy Tamayose
Linha de fomento: Bolsas no Brasil - Doutorado