Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from Crotalus durissus terrificus by Hispidulin from Moquiniastrum floribundum

Autor(es)

dos Santos Junior, Adeilso Bispo ^[1] ; Tamayose, Cinthia I. ^[2] ; Ferreira, Marcelo J. P. ^[2] ; Belchor, Mariana N. ^[1] ; Costa, Caroline R. C. ^[1] ; de Oliveira, Marcos Antonio ^[3] ; Toyama, Marcos Hikari ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biociencias, BIOMOLPEP Grp, Campus Litoral Paulista, BR-11380972 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo 05508090 - Brazil

^[3] UNESP, Inst Biociencias, LABIMES, Campus Litoral Paulista, BR-11380972 Sao Vicente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 25, n. 2 JAN 2 2020.
• Citações Web of Science: 0

Resumo

Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on Crotalus durissus terrificus sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the Moquiniastrum floribundum leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from Crotalus durissus terrificus. (AU)

• Processo FAPESP: 17/20291-0 - Caracterização da atividade pró-inflamatória de uma nova serino protease (cdtsp-2) purificada do veneno total de Crotalus durissus terrificus
• Beneficiário: Marcos Hikari Toyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/21593-2 - Baccharis L. (Asteraceae) e fungos endofíticos associados: metabólitos secundários e atividades biológicas
• Beneficiário: Marcelo José Pena Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/19942-7 - Busca de inibidores do sistema peroxirredoxina de patógenos e humanos
• Beneficiário: Marcos Antonio de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/20932-8 - ESTUDO FITOQUÍMICO, QUIMIOSSISTEMÁTICO E ATIVIDADES BIOLÓGICAS DE ESPÉCIES DE Moquiniastrum E Richterago
• Beneficiário: Cinthia Indy Tamayose
• Modalidade de apoio: Bolsas no Brasil - Doutorado