Amphipathic design dictates self-assembly, cytotoxicity and cell uptake of arginine-rich surfactant-like peptides

Autor(es)

Mello, Lucas R. ^[1] ; Aguiar, Rodrigo B. ^[1] ; Yamada, Renata Y. ^[1] ; Moraes, Jane Z. ^[1] ; Hamley, Ian W. ^[2] ; Alves, Wendel A. ^[3] ; Reza, Mehedi ^[4] ; Ruokolainen, Janne ^[4] ; Silva, Emerson R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biofis, BR-04044020 Sao Paulo - Brazil

^[2] Univ Reading, Dept Chem, Reading RG6 6AD, Berks - England

^[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

^[4] Aalto Univ, Nanomicroscopy Ctr, Puumiehenkuja 2, FIN-02150 Espoo - Finland

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS CHEMISTRY B; v. 8, n. 12, p. 2495-2507, MAR 28 2020.
• Citações Web of Science: 0

Resumo

Amphiphilicity is the most critical parameter in the self-assembly of surfactant-like peptides (SLPs), regulating the way by which hydrophobic attraction holds peptides together. Its effects go beyond supramolecular assembly and may also trigger different cell responses of bioactive peptide-based nanostructures. Herein, we investigate the self-assembly and cellular effects of nanostructures based on isomeric SLPs composed by arginine (R) and phenylalanine (F). Two amphipathic designs were studied: a diblock construct F4R4 and its bolaamphiphile analog R2F4R2. A strong sequence-dependent polymorphism emerges with appearance of globules and vesicle-like assemblies, or flat nanotapes and cylindrical micelles. The diblock construct possesses good cell penetrating capabilities and effectiveness to kill SK-MEL-28 melanoma tumor cells, in contrast to reduced intracellular uptake and low cytotoxicity exhibited by the bolaamphiphilic form. Our findings demonstrate that amphipathic design is a relevant variable for self-assembling SLPs to modulate different cellular responses and may assist in optimizing the production of nanostructures based on arginine-enriched sequences in cell penetrating and antimicrobial peptides. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
• Beneficiário: Adalberto Fazzio
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/24409-3 - Peptídeos Penetrantes de Células para Transporte de DNA plasmídial e microRNA: da Estrutura Nanoscópica a Entrega de Genes.
• Beneficiário: Emerson Rodrigo da Silva
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção
• Beneficiário: Wendel Andrade Alves
• Linha de fomento: Auxílio à Pesquisa - Regular