| Full text | |
| Author(s): |
Mello, Lucas R.
[1]
;
Aguiar, Rodrigo B.
[1]
;
Yamada, Renata Y.
[1]
;
Moraes, Jane Z.
[1]
;
Hamley, Ian W.
[2]
;
Alves, Wendel A.
[3]
;
Reza, Mehedi
[4]
;
Ruokolainen, Janne
[4]
;
Silva, Emerson R.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biofis, BR-04044020 Sao Paulo - Brazil
[2] Univ Reading, Dept Chem, Reading RG6 6AD, Berks - England
[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil
[4] Aalto Univ, Nanomicroscopy Ctr, Puumiehenkuja 2, FIN-02150 Espoo - Finland
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF MATERIALS CHEMISTRY B; v. 8, n. 12, p. 2495-2507, MAR 28 2020. |
| Web of Science Citations: | 4 |
| Abstract | |
Amphiphilicity is the most critical parameter in the self-assembly of surfactant-like peptides (SLPs), regulating the way by which hydrophobic attraction holds peptides together. Its effects go beyond supramolecular assembly and may also trigger different cell responses of bioactive peptide-based nanostructures. Herein, we investigate the self-assembly and cellular effects of nanostructures based on isomeric SLPs composed by arginine (R) and phenylalanine (F). Two amphipathic designs were studied: a diblock construct F4R4 and its bolaamphiphile analog R2F4R2. A strong sequence-dependent polymorphism emerges with appearance of globules and vesicle-like assemblies, or flat nanotapes and cylindrical micelles. The diblock construct possesses good cell penetrating capabilities and effectiveness to kill SK-MEL-28 melanoma tumor cells, in contrast to reduced intracellular uptake and low cytotoxicity exhibited by the bolaamphiphilic form. Our findings demonstrate that amphipathic design is a relevant variable for self-assembling SLPs to modulate different cellular responses and may assist in optimizing the production of nanostructures based on arginine-enriched sequences in cell penetrating and antimicrobial peptides. (AU) | |
| FAPESP's process: | 15/24018-1 - Polymer-Peptide Conjugates for hydrogel formulation and its usage in detection |
| Grantee: | Wendel Andrade Alves |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties |
| Grantee: | Adalberto Fazzio |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/24409-3 - Cell-Penetrating Peptides for Transport of Plasmid DNA and microRNA: from Nanoscopic Structure to Gene Delivery. |
| Grantee: | Emerson Rodrigo da Silva |
| Support Opportunities: | Regular Research Grants |