Assembly and antifungal effect of a new fluconazole-carrier nanosystem

Autor(es)

de Lima, Taynara M. T. ^[1] ; Arias, Lais S. ^[2] ; Afanaci, Leticia F. ^[3] ; Ferraresse, Raphael F. B. ^[3] ; Neto, Francisco N. ^[2] ; de Lima, Bruno H. R. ; Straioto, Fabiana G. ^[1] ; de Camargo, Emerson R. ^[4] ; Pessan, Juliano P. ^[2] ; Monteiro, Douglas R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Western Sao Paulo UNOESTE, Grad Program Dent GPD Masters Degree, BR-19050920 Presidente Prudente Sao - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Dept Prevent & Restorat Dent, BR-16015050 Aracatuba Sao Paulo - Brazil

^[3] Univ Western Sao Paulo UNOESTE, Sch Dent, BR-19050920 Presidente Prudente Sao - Brazil

^[4] Fed Univ Sao Carlos UFSCar, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 15, n. 4, p. 273-286, MAR 2020.
• Citações Web of Science: 5

Resumo

Aim: To assemble, characterize and assess the antifungal effects of a new fluconazole (FLZ)-carrier nanosystem. Materials \& methods: The nanosystem was prepared by loading FLZ on chitosan (CS)-coated iron oxide nanoparticles (IONPs). Antifungal effects were evaluated on planktonic cells (by minimum inhibitory concentration determination) and on biofilms (by quantification of cultivable cells, total biomass, metabolism and extracellular matrix) of Candida albicans and Candida glabrata. Results: Characterization results ratified the formation of a nanosystem (<320 nm) with FLZ successfully embedded. IONPs-CS-FLZ nanosystem reduced minimum inhibitory concentration values and, in general, showed similar antibiofilm effects compared with FLZ alone. Conclusion: IONPs-CS-FLZ nanosystem was more effective than FLZ mainly in inhibiting Candida planktonic cells. This nanocarrier has potential to fight fungal infections. (AU)

• Processo FAPESP: 17/24416-2 - Síntese e efeito antibiofilme de dois novos nanosistemas carreadores de drogas antifúngicas
• Beneficiário: Douglas Roberto Monteiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/08976-8 - Efeito antifúngico de um nanosistema carreador de fluconazol sobre Candida albicans e Candida glabrata
• Beneficiário: Letícia Facholi Afanaci
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/08980-5 - Efeito de um nanosistema carreador de fluconazol na composição da matriz extracelular e estrutura de biofilmes de espécies de Candida
• Beneficiário: Raphael Fernando Barion Ferraresse
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica