Assembly and antifungal effect of a new fluconazole-carrier nanosystem

Author(s)

de Lima, Taynara M. T. ^[1] ; Arias, Lais S. ^[2] ; Afanaci, Leticia F. ^[3] ; Ferraresse, Raphael F. B. ^[3] ; Neto, Francisco N. ^[2] ; de Lima, Bruno H. R. ; Straioto, Fabiana G. ^[1] ; de Camargo, Emerson R. ^[4] ; Pessan, Juliano P. ^[2] ; Monteiro, Douglas R. ^[1]

Affiliation

^[1] Univ Western Sao Paulo UNOESTE, Grad Program Dent GPD Masters Degree, BR-19050920 Presidente Prudente Sao - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Dept Prevent & Restorat Dent, BR-16015050 Aracatuba Sao Paulo - Brazil

^[3] Univ Western Sao Paulo UNOESTE, Sch Dent, BR-19050920 Presidente Prudente Sao - Brazil

^[4] Fed Univ Sao Carlos UFSCar, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 15, n. 4, p. 273-286, MAR 2020.
• Web of Science Citations: 5

Abstract

Aim: To assemble, characterize and assess the antifungal effects of a new fluconazole (FLZ)-carrier nanosystem. Materials \& methods: The nanosystem was prepared by loading FLZ on chitosan (CS)-coated iron oxide nanoparticles (IONPs). Antifungal effects were evaluated on planktonic cells (by minimum inhibitory concentration determination) and on biofilms (by quantification of cultivable cells, total biomass, metabolism and extracellular matrix) of Candida albicans and Candida glabrata. Results: Characterization results ratified the formation of a nanosystem (<320 nm) with FLZ successfully embedded. IONPs-CS-FLZ nanosystem reduced minimum inhibitory concentration values and, in general, showed similar antibiofilm effects compared with FLZ alone. Conclusion: IONPs-CS-FLZ nanosystem was more effective than FLZ mainly in inhibiting Candida planktonic cells. This nanocarrier has potential to fight fungal infections. (AU)

• FAPESP's process: 17/24416-2 - Synthesis and antibiofilm effect of two new carrier nanosystems of antifungal drugs
• Grantee: Douglas Roberto Monteiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/08976-8 - Antifungal effect of a fluconazole carrier nanosystem on Candida albicans and Candida glabrata
• Grantee: Letícia Facholi Afanaci
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/08980-5 - Effect of a fluconazole carrier nanosystem on the extracellular matrix composition and structure of Candida biofilms
• Grantee: Raphael Fernando Barion Ferraresse
• Support Opportunities: Scholarships in Brazil - Scientific Initiation