Nano-in-nano containing curcumin and benzydamine hydrochloride for the treatment of Vulvovaginal Candidiasis: from development to biological application in vitro and in vivo

• Grant number: 19/26821-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2021
• End date: July 31, 2024
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Marlus Chorilli
• Grantee: Gabriela Corrêa Carvalho
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 22/02187-0 - Nano-in-nano system containing curcumin and benzydamine hydrochloride for the treatment of vulvovaginal candidiasis: from development to in vitro anti-inflammatory application, BE.EP.DR

Abstract

Vulvovaginal candidiasis currently represents a major public health challenge due to its high incidence and reports of recurrences. These challenges are due to host-related factors, such as a compromised immune system, or pathogen-related factors, including resistance to antifungal agents, making it necessary to study new treatment alternatives, for example, substances of natural origin, such as curcumin (CUR). However, this substance presents problems that limit its therapeutic application, such as insolubility in aqueous solvents, which leads to low bioavailability. Despite this, the association of nanotechnology and drugs of plant origin proves to be a promising alternative to overcome the aforementioned limitations. Although this pathology is caused by a fungus, patients suffer a lot from the inflammation caused by it, therefore, this proposal aims to combine two drugs, benzydamine hydrochloride (anti-inflammatory) and curcumin (anti-fungal) in one system. nano-in-nano consisting of mesoporous silica nanoparticles (MSN) in a lipid structure (liposome). These two nanosystems were chosen to be combined in a hybrid system in order to combine characteristics such as chemical stability, versatility in the presence of pores, large surface area and hydroxyl groups of NSM with the high biocompatibility of the liposome. Among the different routes of administration, the vaginal route can be advantageous, as the vaginal mucosa has high permeability, high blood supply and lack of first-rate metabolism passage, optimizing the local action. Furthermore, the dispersion of the nano-in-nano system in thermo-responsive hydrogels would not only facilitate the application of the formulation in the vagina, since it is liquid at room temperature and gel at body temperature, but would also increase its residence time in the vagina environment, allowing sustained release at the specific site of action. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, GABRIELA CORREA; FURQUIM DE CAMARGO, BRUNA ALMEIDA; CAVALCANTE DE ARAUJO, JENNIFER THAYANNE; CHORILLI, MARLUS. Lycopene: From tomato to its nutraceutical use and its association with nanotechnology. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 118, p. 447-458, DEC 2021. (19/26821-7, 20/06212-3)

CARVALHO, GABRIELA CORREA; MARENA, GABRIEL DAVI; FISCHER KARNOPP, JULIANA CAMILA; JORGE, JULIANA; SABIO, RAFAEL MIGUEL; UTRERA MARTINES, MARCO ANTONIO; BAUAB, TAIS MARIA; CHORILLI, MARLUS. Cetyltrimethylammonium bromide in the synthesis of mesoporous silica nanoparticles: General aspects and in vitro toxicity. ADVANCES IN COLLOID AND INTERFACE SCIENCE, v. 307, p. 14-pg., 2022-08-12. (19/26821-7, 18/25377-3, 19/09831-9)

CARVALHO, GABRIELA CORREA; MARENA, GABRIEL DAVI; LEONARDI, GABRIELA RICCI; SABIO, RAFAEL MIGUEL; CORREA, IONE; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Lycopene, Mesoporous Silica Nanoparticles and Their Association: A Possible Alternative against Vulvovaginal Candidiasis?. Molecules, v. 27, n. 23, p. 13-pg., 2022-12-01. (19/26821-7, 18/25377-3, 19/09831-9)

MARENA, GABRIEL DAVI; DOS SANTOS RAMOS, MATHEUS APARECIDO; CARVALHO, GABRIELA CORREA; DE LIMA, LAURA CAMINITTI; CARNEIRO SOARES DO NASCIMENTO, ANDRE LUIZ; SABIO, RAFAEL MIGUEL; RODERO, CAMILA FERNANDA; SPOSITO, LARISSA; BAUAB, TAIS MARIA; CHORILLI, MARLUS. Development and characterization of an amphotericin B - loaded nanoemulsion applied to Candida auris biofilms control. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 74, p. 14-pg., 2022-07-20. (19/26821-7, 18/25377-3, 19/09597-6, 19/09831-9, 18/23442-2, 14/50928-2, 17/22758-3, 18/23357-5)

MARENA, G. D.; CARVALHO, G. C.; MONAZZI, L. C. S.; MASCHIO-LIMA, T.; DE ALMEIDA, M. T. G.; DA SILVA, J. L. M.; FORTUNATO, G. C.; ARAUJO, V. H. S.; VENANCIO, D. C. V.; CHANG, M. R.; et al. Infection caused by Candida auris: state of the art. MYCOSPHERE, v. 13, n. 1, p. 42-pg., 2022-01-01. (19/09831-9, 19/26821-7)

RIBEIRO, TAIS C.; SABIO, RAFAEL M.; CARVALHO, GABRIELA C.; FONSECA-SANTOS, BRUNO; CHORILLI, MARLUS. Exploiting mesoporous silica, silver and gold nanoparticles for neurodegenerative diseases treatment. International Journal of Pharmaceutics, v. 624, p. 16-pg., 2022-07-07. (19/26821-7, 19/19228-8, 18/25377-3)

MARENA, GABRIEL DAVI; CARVALHO, GABRIELA CORREA; RAMOS, MATHEUS APARECIDO DOS SANTOS; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Anti-Candida auris activity in vitro and in vivo of micafungin loaded nanoemulsions. Medical Mycology, v. 61, n. 2, p. 11-pg., 2023-02-03. (19/26821-7, 19/09831-9, 14/50928-2, 18/23442-2)

ALMEIDA FURQUIM DE CAMARGO, BRUNA; FONSECA-SANTOS, BRUNO; GONCALVES CARVALHO, SUZANA; CORREA CARVALHO, GABRIELA; DELELLO DI FILIPPO, LEONARDO; SOUSA ARAUJO, VICTOR HUGO; LOBATO DUARTE, JONATAS; POLLI SILVESTRE, AMANDA LETICIA; BAUAB, TAIS MARIA; CHORILLI, MARLUS. Functionalized lipid-based drug delivery nanosystems for the treatment of human infectious diseases. CRITICAL REVIEWS IN MICROBIOLOGY, v. N/A, p. 17-pg., 2022-05-28. (19/26821-7, 19/10261-2)

CARVALHO, GABRIELA CORREA; SABIO, RAFAEL MIGUEL; SPOSITO, LARISSA; ANDREOLI PINTO, TEREZINHA DE JESUS; CHORILLI, MARLUS. An overview of the use of central venous catheters impregnated with drugs or with inorganic nanoparticles as a strategy in preventing infections. International Journal of Pharmaceutics, v. 615, p. 12-pg., 2022-03-05. (19/26821-7, 18/25377-3)