Syngonanthus nitens (Bong.) Ruhland: prospection chemico-biologic of methanolic extract of scapes in the treatment of vuvlvovaginal candidiasis.

• Grant number: 14/24626-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2016
• End date: January 31, 2019
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Taís Maria Bauab
• Grantee: Matheus Aparecido dos Santos Ramos
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 16/17109-3 - Interference of Syngonanthus nitens (Bong.) Ruhland in the adhesion mechanisms of Candida albicans biofilms, BE.EP.DR

Abstract

The indiscriminate and wide spread use of antifungal drugs took to the development of strains resistant to drugs used in clinical therapy, thischaracterized as themain cause of numerous cases of inappropriate patient to drug therapy available. Natural products are gaining prominence by providing a new pharmacological approach for the treatment of pathological processes, among these, arising from infectious Candida yeasts. The virulence profiles assigned to microorganisms of this genus are related to the production of hyphae in host tissues, phosphatases and hemolysin production and especially the production of biofilm. The adherence to tissues (biofilm) ranks as one aggravating factor in therapy, especially when it is in conjunction with other micro-organisms (heterotypic biofilms), especially pathogenic bacteria like Lactobacillus sp. The pharmaceutical nanotechnology presents it self as an important tool to improve the bioavailability of natural products, while offering modern drug systems, employees for placement of plant extracts. Plants belonging to family Eriocaulaceae show promise in antifungal activity, which encourages the deepening of these investigations, as well as the discovery of new methods of applicability. This study aims to evaluate the antifungal potential of fractions of the methanol extract of Syngonanthus nitens scapes, which has proved promising in the treatment of VVC, incorporated and not incorporated into nanostructured drug delivery system (lipid microemulsion) aiming to improve the parameters for bioavailability there of. Analysis will be employed for antifungal focusing on monotypical biofilms (Candida albicans) and heterotypic (Candida albicans + Lactobacillus sp) by using in vitro assays, in vivo assays, flow chamber (Chamber Flow Technique) testing and residence time, beyond the elucidation of the mechanisms of action by transcriptomic analysis. It is hoped to obtain important results concerning the capability of the plant to compose the armamentarium of treatment of VVC.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SPOSITO, LARISSA; DE TOLEDO, LUCIANI GASPAR; BONIFACIO, BRUNA VIDAL; RODERO, CAMILA FERNANDA; DOS SANTOS, KAREN CRISTINA; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanotechnology-based drug delivery systems for control of microbial biofilms: a review. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 13, p. 1179-1213, 2018. (16/08559-5, 14/24626-9)

DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; DE TOLEDO, LUCIANI GASPAR; ODA, FERNANDO BOMBARDA; DA SILVA, ISABEL CRISTIANE; DOS SANTOS, LOURDES CAMPANER; DOS SANTOS, ANDRE GONZAGA; GOTTARDO DE ALMEIDA, MARGARETE TERESA; PAVAN, FERNANDO ROGERIO; CHORILLI, MARIUS; et al. Intravaginal Delivery of Syngonanthus nitens (Bong.) Ruhland Fraction Based on a Nanoemulsion System Applied to Vulvovaginal Candidiasis Treatment. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 15, n. 5, p. 1072-1089, MAY 2019. (14/24626-9, 16/08559-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RAMOS, Matheus Aparecido dos Santos. Syngonanthus nitens (Bong.) Ruhland: prospecção químico-biológica do extrato metanólico de escapos no tratamento da candidíase vulvovaginal. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.