Microemulsion co-delivering vitamin A and vitamin E as a new platform for topical treatment of acute skin inflammation

Autor(es)

Praca, Pablola Garcia ^[1] ; Rosa Viegas, Juliana Santos ^[1] ; Peh, Hong Yong ^[2] ; Garbin, Tuane Nardacchione ^[3] ; Garcia Medina, Wanessa Silva ^[3] ; Lopes Badra Bentley, Maria Vitoria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Ribeirao Preto, SP - Brazil

^[2] Harvard Med Sch, Brigham & Womens Hosp, Dept Internal Med, Div Pulm & Crit Care Med, Boston, MA 02115 - USA

^[3] Ctr Univ Padre Albino UNIFIPA, Catanduva, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 110, MAY 2020.
• Citações Web of Science: 0

Resumo

In this study, we developed a water-in-oil microemulsion containing vitamin A (retinol) and vitamin E (alpha-tocopherol), which serves as a multifunctional nanosystem that co-delivers antioxidants and displayed additive effect against acute skin inflammation. Microemulsion (ME) was prepared by mixing a surfactant blend (Tween 80 and propylene glycol, 5:1) with isopropyl myristate and water (ratio of 50:40:10, respectively). Vitamin A (0.05% w/w concentration) and/or vitamin E (0.1% w/w concentration) were incorporated into the surfactant mixture of ME by stirring with a magnetic stirrer for 30 min. This multifunctional ME displayed physical stability, with low cytotoxicity in 3T3 cell line, as well as cellular internalization into the cytosol. In vivo treatments using ME delivering alpha-tocopherol reduced dermal expression of TNF-alpha by 1.3-fold (p < 0.01), when compared to unloaded ME treatment group. When retinol was added into the ME containing alpha-tocopherol, it further reduced TNF-alpha expression by 2-fold (p < 0.001), suggesting the additive effect of vitamin E and vitamin A in the treatment against skin inflammation. In conclusion, we successfully developed the use of water-in-oil ME to pack both vitamin E and vitamin A, and demonstrated for the first time its anti-inflammatory potential when applied topically to TPA-induced inflamed skin. (AU)

• Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Modalidade de apoio: Auxílio à Pesquisa - Temático