| Texto completo | |
| Autor(es): |
de Lima Benzi, Jhohann Richard
[1]
;
Moreira, Fernanda de Lima
[1]
;
Marques, Maria Paula
[1]
;
Duarte, Geraldo
[2]
;
Suarez-Kurtz, Guilherme
[3]
;
Lanchote, Vera Lucia
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Ave Cafe S-N, Campus USP, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[3] Natl Canc Inst, Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1144, MAY 1 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Different methods have been used for CYP3A phenotyping, such as probe drugs or the urinary index 6 beta-hydroxycortisol/cortisol ratio (6 beta-OHF:C). This work describes a simple and affordable method for the simultaneous determination of the endogenous compounds cortisol and 6 beta-hydroxycortisol in urine using a background subtraction approach. The method was applied to investigate the CYP3A activity in HIV-infected pregnant women (n = 9) in the third trimester and postpartum periods. Also, the within-day variability in the 6 ss-OHF:C index was also evaluated. The sample preparation consists of a pre-cleanup with acetonitrile followed by liquidliquid extraction with ethyl acetate. The analytes were resolved by employing an Acquity UPLC (R) BEH C18 column with a mobile phase that consisted of a mixture of acetonitrile containing 0.1% formic acid and 0.1% formic acid in gradient mode. The method presented linearities of 1-1.000 ng/mL and 2-1.000 ng/mL for C and 6 beta-OHF, respectively, and presented acceptable precision and accuracy. Qualitative and quantitative matrix effects tests were also performed. A high 6 beta-OHF:C within-day variability was observed in both phases. In the third trimester period, the 6 beta-OHF:C ranged from 2.57 to 51.69, with a mean +/- standard deviation (SD) of 15.12 +/- 5.41 (n = 9). Similar values were obtained in the postpartum period, with 6 beta-OHF:C ranging from 3.48 to 44.54 with a mean +/- SD of 14.37 +/- 5.73 (n = 7). Even though the 6 beta-OHF:C is a non-invasive index for CYP3A phenotyping, its use is susceptible to high within-day variability. (AU) | |
| Processo FAPESP: | 19/03429-4 - Impacto da Pielonefrite na atividade dos transportadores de ânions orgânicos (OAT) 1 e 3 em gestantes: uma abordagem quantitativa da farmacologia dos sistemas |
| Beneficiário: | Jhohann Richard de Lima Benzi |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/05616-3 - Farmacocinética clínica em doenças infecciosas |
| Beneficiário: | Vera Lúcia Lanchote |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/23938-2 - Influência da gravidez associada com HIV na atividade do transportador OATP em pacientes em tratamento com Efavirenz |
| Beneficiário: | Fernanda de Lima Moreira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |