| Texto completo | |
| Autor(es): |
Sant'Ana, Monielle
[1]
;
Souza, Helena R.
[2, 3]
;
Possebon, Lucas
[2, 3]
;
Cornelio, Marinonio L.
[4]
;
Riffo-Vasquez, Yanira
[5]
;
Girol, Ana Paula
[2, 3]
;
Oliani, Sonia M.
[3, 1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Sao Paulo Fed Univ, Dept Morphol & Genet, UNIFESP, Sao Paulo, SP - Brazil
[2] Univ Ctr Padre Albino UNIFIPA, Catanduva, SP - Brazil
[3] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci IBILCE, Dept Biol, UNESP, Lab Immunomorphol, Sao Jose Do Rio Preto Campus, Sao Jose Do Rio Preto, SP - Brazil
[4] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci IBILCE, Dept Phys, UNESP, Sao Jose Do Rio Preto Campus, Sao Jose Do Rio Preto, SP - Brazil
[5] Kings Coll London, Sackler Inst Pulm Pharmacol, Inst Pharmaceut Sci, London - England
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Pulmonary Pharmacology & Therapeutics; v. 61, APR 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Chronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke. (AU) | |
| Processo FAPESP: | 16/02012-4 - Investigação das atividades imunomoduladoras da proteína anexina A1 na regulação inflamatória de doenças do sistema gastrointestinal: estudo em modelos experimentais in vivo e in vitro |
| Beneficiário: | Sonia Maria Oliani |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/13041-4 - Avaliação de transplantes heterólogos de pele usando matrizes acelulares obtidas de pele suína e ação da proteína anexina A1 como agente anti-inflamatório/imunossupressor |
| Beneficiário: | Kallyne Kioko Oliveira Mimura |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |