Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities

Autor(es)

dos Santos, Catarina ^[1] ; Mizobucchi, Andressa Lie ^[1] ; Escaramboni, Bruna ^[2] ; Lopes, Bruno Pereira ^[3] ; Figueiredo Angolini, Celio Fernando ^[4] ; Eberlin, Marcos Nogueira ^{[5, 6]} ; de Toledo, Karina Alves ^[3] ; Fernandez Nunez, Eutimio Gustavo ^[7]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, Fac Sci & Letters, Dept Biol Sci, Lab Chem UNESP Assis LAQUA, UNESP, Sto Antonio Ave, BR-19806900 Assis, SP - Brazil

^[2] Univ Sao Paulo State, Fac Sci & Letters, Dept Biotechnol, UNESP, BR-19806900 Assis, SP - Brazil

^[3] Univ Sao Paulo State, Fac Sci & Letters, Dept Biol Sci, Lab Physiopathol Innate Immun, UNESP, BR-19806900 Assis, SP - Brazil

^[4] Fed Univ ABC UFABC, Ctr Nat & Human Sci, Mass Spectrometry & Chem Ecol Lab MS Cell, Sao Paulo, SP - Brazil

^[5] Univ Estadual Campinas, Inst Chem, Thomson Mass Spectrometry Lab, UNICAMP, Sao Paulo - Brazil

^[6] Univ Prebiteriana Mackenzie, Mackenzie Res Nucleus Sci Faith & Soc, 163 Room 44, BR-01222010 Sao Paulo, SP - Brazil

^[7] Univ Sao Paulo EACH USP, Sch Arts Sci & Humanities, Arlindo Bettio St 1000, BR-03828000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC CHEMISTRY; v. 14, n. 1 APR 27 2020.
• Citações Web of Science: 0

Resumo

Eugenia punicifolia (Kunth) D. C. (Myrtaceae) has been showing interesting biological activities in the literature which was correlated to its phenolic compounds. In the sense of a better recovering of phenolics with the best antioxidant and antiproliferative activities, an extraction, based on multivariate analytical approach, was developed from E. punicifolia leaves. The different extractor solvents (ethanol, methanol and water) and their binary and ternary combinations were evaluated using a simplex-centroid mixture design and surface response methodology. The optimized crude extracts were investigated for phenol and flavonoid content and compared to their antioxidant (EC50) and antiproliferative properties against HEp-2 (cell line derived from the oropharyngeal carcinoma) and mononuclear viability cells. Ethanolic extracts showed the best phenolic content with the highest antioxidant activity and moderated activity antiproliferative to HEp-2. ESI-QTOF-MS revealed the presence of quercetin and myricetin derivatives, which was correlated to activities tested. Then, simplex-centroid design allowed us to correlate the Eugenia punicifolia biological activities with the extracts obtained from solvent different polarity mixtures. (AU)

• Processo FAPESP: 14/19504-1 - Otimização da mistura de solventes para extração de compostos fenólicos com atividade antioxidante e antiproliferativa das folhas de Eugenia punicifolia (Kunth) DC por aplicação do delineamento simplex-centroide
• Beneficiário: Andressa Lie Mizobuchi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/15610-0 - Potencialidades biológicas em Myrtaceae
• Beneficiário: Catarina dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular