Enzyme Immobilization on Maghemite Nanoparticles with Improved Catalytic Activity: An Electrochemical Study for Xanthine

Autor(es)

Magro, Massimiliano ^[1] ; Baratella, Davide ^[1] ; Venerando, Andrea ^[1] ; Nalotto, Giulia ^[1] ; Basso, Caroline R. ^[2] ; Molinari, Simone ^[3] ; Salviulo, Gabriella ^[3] ; Ugolotti, Juri ^[4] ; Pedrosa, Valber A. ^[2] ; Vianello, Fabio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Padua Agripolis, Dept Comparat Biomed & Food Sci, Viale Univ 16, Legnaro 35020, PD - Italy

^[2] Univ Estadual Paulista, Dept Chem & Biochem, Inst Biosci, Botucatu 18618000, SP - Brazil

^[3] Univ Padua, Dept Geosci, Via G Gradenigo 6, Padua 35131 - Italy

^[4] Palacky Univ Olomouc, Reg Ctr Adv Technol & Mat, Slechtitelu 27, Olomouc 78371 - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 13, n. 7 APR 2020.
• Citações Web of Science: 0

Resumo

Generally, enzyme immobilization on nanoparticles leads to nano-conjugates presenting partially preserved, or even absent, biological properties. Notwithstanding, recent research demonstrated that the coupling to nanomaterials can improve the activity of immobilized enzymes. Herein, xanthine oxidase (XO) was immobilized by self-assembly on peculiar naked iron oxide nanoparticles (surface active maghemite nanoparticles, SAMNs). The catalytic activity of the nanostructured conjugate (SAMN@XO) was assessed by optical spectroscopy and compared to the parent enzyme. SAMN@XO revealed improved catalytic features with respect to the parent enzyme and was applied for the electrochemical studies of xanthine. The present example supports the nascent knowledge concerning protein conjugation to nanoparticle as a means for the modulation of biological activity. (AU)

• Processo FAPESP: 19/20573-1 - Utilização de nanopartículas para desenvolvimento de kits de quantificação do Circovírus suíno 2 (PCV-2)
• Beneficiário: Caroline Rodrigues Basso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/13411-5 - Desenvolvimento de dispositivo microfluídico para biopsia líquida de células tumorais circulantes
• Beneficiário: Valber de Albuquerque Pedrosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular