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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enzyme Immobilization on Maghemite Nanoparticles with Improved Catalytic Activity: An Electrochemical Study for Xanthine

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Author(s):
Magro, Massimiliano [1] ; Baratella, Davide [1] ; Venerando, Andrea [1] ; Nalotto, Giulia [1] ; Basso, Caroline R. [2] ; Molinari, Simone [3] ; Salviulo, Gabriella [3] ; Ugolotti, Juri [4] ; Pedrosa, Valber A. [2] ; Vianello, Fabio [1]
Total Authors: 10
Affiliation:
[1] Univ Padua Agripolis, Dept Comparat Biomed & Food Sci, Viale Univ 16, Legnaro 35020, PD - Italy
[2] Univ Estadual Paulista, Dept Chem & Biochem, Inst Biosci, Botucatu 18618000, SP - Brazil
[3] Univ Padua, Dept Geosci, Via G Gradenigo 6, Padua 35131 - Italy
[4] Palacky Univ Olomouc, Reg Ctr Adv Technol & Mat, Slechtitelu 27, Olomouc 78371 - Czech Republic
Total Affiliations: 4
Document type: Journal article
Source: MATERIALS; v. 13, n. 7 APR 2020.
Web of Science Citations: 0
Abstract

Generally, enzyme immobilization on nanoparticles leads to nano-conjugates presenting partially preserved, or even absent, biological properties. Notwithstanding, recent research demonstrated that the coupling to nanomaterials can improve the activity of immobilized enzymes. Herein, xanthine oxidase (XO) was immobilized by self-assembly on peculiar naked iron oxide nanoparticles (surface active maghemite nanoparticles, SAMNs). The catalytic activity of the nanostructured conjugate (SAMN@XO) was assessed by optical spectroscopy and compared to the parent enzyme. SAMN@XO revealed improved catalytic features with respect to the parent enzyme and was applied for the electrochemical studies of xanthine. The present example supports the nascent knowledge concerning protein conjugation to nanoparticle as a means for the modulation of biological activity. (AU)

FAPESP's process: 19/20573-1 - Use of nanoparticles to development of kits for Porcine Circoviruses 2 (PCV-2) quantification
Grantee:Caroline Rodrigues Basso
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/13411-5 - Development of microfluidic device for liquid biopsy of circulating tumor cells
Grantee:Valber de Albuquerque Pedrosa
Support Opportunities: Regular Research Grants