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Preparation and characterization of Bifunctional-Nanostructured surfaces of Au-Pt obtained via templates for application in sensors and biosensors

Grant number: 12/17689-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2012
Effective date (End): April 30, 2016
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Sergio Antonio Spinola Machado
Grantee:Paulo Augusto Raymundo Pereira
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

In the present project proposes to develop and characterize bifunctional surfaces, composed of gold nanoparticles electrodeposited on platinum assisted by templates and coated with layers of self-assembled alkanethiols for the preparation of amperometric biosensors. The surface of polycrystalline platinum electrode is initially modified by electrodeposition of Au nanostructures assisted by template, electrodeposited from an electrolyte containing tetracloroaurato by linear sweep voltammetry. These modified surfaces are characterized by electrochemical techniques (cyclic voltammetry, impedance spectroscopy) and techniques of atomic force microscopy to determine the size and distribution of the gold nanoparticles on the surface, and the exposed area of platinum. Due to the considerable increase in the electroactive area of the electrode test will be electrocatalytic activity of the modified electrode with respect to the compounds of analytical interest. In a second step, the gold nanoparticles are coated with layers of self-assembled alkanethiol (cystamine) aimed at preparing the surface for the immobilization of the enzyme. This coverage will be analyzed by electrochemical techniques (cyclic voltammetry and electrochemical impedance spectroscopy) and by atomic force microscopy. Finally, the layer of self-organized cystamine is immobilized enzyme glucose oxidase, lactate oxidase, and cholesterol oxidase to prepare a biosensor. The product of the enzymatic reaction, hydrogen peroxide (H2O2) will be determined in the platinum surface, with the goal of using the catalytic properties of this surface to improve the sensitivity of the biosensor.

Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMPOS, ANDERSON M.; RAYMUNDO-PEREIRA, PAULO A.; MENDONCA, CAMILA D.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N. Size Control of Carbon Spherical Shells for Sensitive Detection of Paracetamol in Sweat, Saliva, and Urine. ACS APPLIED NANO MATERIALS, v. 1, n. 2, p. 654-661, FEB 2018. Web of Science Citations: 4.
RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; VICENTINI, FERNANDO C.; JANEGITZ, BRUNO C.; MENDONCA, CAMILA D.; FURINI, LEONARDO N.; BOAS, NAIZA V.; CALEGARO, MARCELO L.; CONSTANTINO, CARLOS J. L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N. Sensitive detection of estriol hormone in creek water using a sensor platform based on carbon black and silver nanoparticles. Talanta, v. 174, p. 652-659, NOV 1 2017. Web of Science Citations: 8.
RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; MENDONCA, CAMILA D.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N. Printex 6L Carbon Nanoballs used in Electrochemical Sensors for Simultaneous Detection of Emerging Pollutants Hydroquinone and Paracetamol. SENSORS AND ACTUATORS B-CHEMICAL, v. 252, p. 165-174, NOV 2017. Web of Science Citations: 21.
RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; COELHO, DYOVANI; PIAZZETA, MARIA H. O.; GOBBI, ANGELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N. A Nanostructured Bifunctional platform for Sensing of Glucose Biomarker in Artificial Saliva: Synergy in hybrid Pt/Au surfaces. BIOSENSORS & BIOELECTRONICS, v. 86, p. 369-376, DEC 15 2016. Web of Science Citations: 17.
RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; PRADO, THIAGO M.; FURINI, LEONARDO N.; BOAS, NAIZA V.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S. Synergy between Printex nano-carbons and silver nanoparticles for sensitive estimation of antioxidant activity. Analytica Chimica Acta, v. 926, p. 88-98, JUL 5 2016. Web of Science Citations: 9.
VICENTINI, FERNANDO CAMPANHA; RAYMUNDO-PEREIRA, PAULO A.; JANEGITZ, BRUNO C.; MACHADO, SERGIO A. S.; FATIBELLO-FILHO, ORLANDO. Nanostructured carbon black for simultaneous sensing in biological fluids. SENSORS AND ACTUATORS B-CHEMICAL, v. 227, p. 610-618, MAY 2016. Web of Science Citations: 30.
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RAYMUNDO PEREIRA, PAULO AUGUSTO; RODRIGUES, VALQUIRIA DA CRUZ; SHIMIZU, FLAVIO MAKOTO; MELENDEZ, MATIAS ELISEO; NETO, CRISTOVAM SCAPULATEMPO; CARVALHO, ANDRE LOPES; LEITE, FABIO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N. Adsorption according to the Langmuir-Freundlich model is the detection mechanism of the antigen p53 for early diagnosis of cancer. Physical Chemistry Chemical Physics, v. 18, n. 12, p. 8412-8418, MAR 28 2016. Web of Science Citations: 16.
RAYMUNDO-PEREIRA, PAULO A.; MASCARENHAS, ANA C. V.; TEIXEIRA, MARCOS F. S. Evaluation of the Oxo-bridged Dinuclear Ruthenium Ammine Complex as Redox Mediator in an Electrochemical Biosensor. Electroanalysis, v. 28, n. 3, p. 562-569, MAR 2016. Web of Science Citations: 2.
RAYMUNDO-PEREIRA, PAULO A.; CECCATO, DIEGO A.; JUNIOR, AIRTON G. B.; TEIXEIRA, MARCOS F. S.; LIMA, SERGIO A. M.; PIRES, ANA. M. Study on the structural and electrocatalytic properties of Ba2+- and Eu3+-doped silica xerogels as sensory platforms. RSC ADVANCES, v. 6, n. 106, p. 104529-104536, 2016. Web of Science Citations: 3.
RAYMUNDO-PEREIRA, PAULO A.; LIMA, ALAN R. F.; MACHADO, SERGIO A. S. A nanostructured label-free platform based on an ultrathin film for ultrasensitive detection of a secosteroid hormone. RSC ADVANCES, v. 6, n. 41, p. 34458-34467, 2016. Web of Science Citations: 5.
RAYMUNDO-PEREIRA, PAULO A.; TEIXEIRA, MARCOS F. S.; CAETANO, FABIO R.; BERGAMINI, MARCIO F.; MARCOLINO-JUNIOR, LUIZ H. A Simple and Rapid Estimation of Totals Polyphenols Based On Carbon Paste Electrode Modified with Ruthenium Oxo-Complex. Electroanalysis, v. 27, n. 10, p. 2371-2376, OCT 2015. Web of Science Citations: 4.
CANEVARI, THIAGO C.; RAYMUNDO-PEREIRA, PAULO A.; LANDERS, RICHARD; BENVENUTTI, EDILSON V.; MACHADO, SERGIO A. S. Sol-gel thin-film based mesoporous silica and carbon nanotubes for the determination of dopamine, uric acid and paracetamol in urine. Talanta, v. 116, p. 726-735, NOV 15 2013. Web of Science Citations: 45.
CANEVARI, THIAGO C.; RAYMUNDO-PEREIRA, PAULO A.; LANDERS, RICHARD; MACHADO, SERGIO A. S. Direct Synthesis of Ag Nanoparticles Incorporated on a Mesoporous Hybrid Material as a Sensitive Sensor for the Simultaneous Determination of Dihydroxybenzenes Isomers. European Journal of Inorganic Chemistry, v. 2013, n. 33, p. 5746-5754, NOV 12 2013. Web of Science Citations: 16.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
Preparation and Characterization of Bifunctional-Nanostructured Surfaces of Pt/Au Obtained Via Template for Application in Sensors and Biosensors. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos São Carlos.

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